diff --git a/actions/waveform-analysis/attributes.yaml b/actions/waveform-analysis/attributes.yaml index 35ae4c621..a55ed0197 100644 --- a/actions/waveform-analysis/attributes.yaml +++ b/actions/waveform-analysis/attributes.yaml @@ -1,7 +1,7 @@ -registered: '2019-10-04T10:30:30' -data: - figures: figures - statistics: statistics - notebook: 10_waveform_analysis.ipynb - html: 10_waveform_analysis.html - results: results.csv +registered: '2019-10-04T10:30:30' +data: + figures: figures + statistics: statistics + notebook: 10_waveform_analysis.ipynb + html: 10_waveform_analysis.html + results: results.csv diff --git a/actions/waveform-analysis/data/10_waveform_analysis.html b/actions/waveform-analysis/data/10_waveform_analysis.html index 144c23ae6..60b2788ef 100644 --- a/actions/waveform-analysis/data/10_waveform_analysis.html +++ b/actions/waveform-analysis/data/10_waveform_analysis.html @@ -1,14921 +1,14479 @@ - - - - -10_waveform_analysis - - - - - - - - - - - - - - - - - - - - - - - -
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%load_ext autoreload
-%autoreload 2
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import os
-import expipe
-import pathlib
-import numpy as np
-import spatial_maps.stats as stats
-import septum_mec
-import septum_mec.analysis.data_processing as dp
-import septum_mec.analysis.registration
-import head_direction.head as head
-import spatial_maps as sp
-import speed_cells.speed as spd
-import re
-import joblib
-import multiprocessing
-import shutil
-import psutil
-import pandas as pd
-import matplotlib.pyplot as plt
-from distutils.dir_util import copy_tree
-from neo import SpikeTrain
-import scipy
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-from tqdm import tqdm_notebook as tqdm
-from tqdm._tqdm_notebook import tqdm_notebook
-tqdm_notebook.pandas()
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-from spike_statistics.core import permutation_resampling
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-from spikewaveform.core import calculate_waveform_features_from_template, cluster_waveform_features
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-from septum_mec.analysis.plotting import violinplot
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%matplotlib inline
-color_control = '#4393c3'
-color_stimulated = '#d6604d'
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-color_bs = '#5aae61'
-color_ns = '#9970ab'
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-figsize_violin = (1.7, 3)
-figsize_gen = (4, 3)
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-output_path = pathlib.Path("output") / "waveform-analysis"
-(output_path / "statistics").mkdir(exist_ok=True, parents=True)
-(output_path / "figures").mkdir(exist_ok=True, parents=True)
-output_path.mkdir(exist_ok=True)
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data_loader = dp.Data()
-actions = data_loader.actions
-project = data_loader.project
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identify_neurons = actions['identify-neurons']
-sessions = pd.read_csv(identify_neurons.data_path('sessions'))
-units = pd.read_csv(identify_neurons.data_path('units'))
-session_units = pd.merge(sessions, units, on='action')
-#########################3
-# session_units = session_units.drop_duplicates('unit_id')
-#################################
-print('N cells:',session_units.shape[0])
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N cells: 1281
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session_units.head()
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actioncontrolentityfrequencysessionstim_locationstimulatedtagchannel_groupunit_name
01849-060319-3True1849NaN3NaNFalsebaseline ii1104
11849-060319-3True1849NaN3NaNFalsebaseline ii1108
21849-060319-3True1849NaN3NaNFalsebaseline ii185
31849-060319-3True1849NaN3NaNFalsebaseline ii194
41849-060319-3True1849NaN3NaNFalsebaseline ii198
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session_units.groupby('action').count().unit_name.hist()
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<matplotlib.axes._subplots.AxesSubplot at 0x7f06055511d0>
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Process all data

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def features(row):
-    action_id = row['action']
-    channel_id = row['channel_group']
-    unit = row['unit_name']
-    template = data_loader.template(action_id, channel_id, unit)
-    spike_times = data_loader.spike_train(action_id, channel_id, unit)
-    half_widths, peak_to_troughs = calculate_waveform_features_from_template(
-        template.data, template.sampling_rate)
-    peak_amps = template.data.min(axis=1)
-    half_widths = half_widths * 1000 # to ms
-    peak_to_troughs = peak_to_troughs * 1000 # to ms
-    idxs = np.argsort(peak_amps)
-    peak_to_trough = np.nan
-    for p2t in peak_to_troughs[idxs]:
-        if np.isfinite(p2t) and p2t > .1:
-            peak_to_trough = p2t
-            break
-    half_width = np.nan
-    for hw in half_widths[idxs]:
-        if np.isfinite(hw):
-            half_width = hw
-            break
-    
-    return pd.Series({
-        'half_width': half_width,
-        'peak_to_trough': peak_to_trough,
-        'average_firing_rate': float(len(spike_times) / spike_times.t_stop),
-        'template': template.data[idxs[0]]
-    })
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results = session_units.merge(
-    session_units.progress_apply(features, axis=1), 
-    left_index=True, right_index=True)
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df = results.loc[:, ['half_width', 'peak_to_trough']].dropna()
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-idxs_df = cluster_waveform_features(df.half_width, df.peak_to_trough)
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-results.loc[df.index, 'bs'] = idxs_df
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plt.figure(figsize=figsize_gen)
-size = 5
-mew = .5
-marker_bs = '.'
-marker_ns = '+'
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-plt.scatter(
-    results.query('bs==0')['half_width'], 
-    results.query('bs==0')['peak_to_trough'], 
-    c=color_ns, s=size, marker=marker_ns, linewidth=mew, label='NS')
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-plt.scatter(
-    results.query('bs==1')['half_width'], 
-    results.query('bs==1')['peak_to_trough'], 
-    c=color_bs, s=size, marker=marker_bs, linewidth=mew, label='BS')
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-plt.xlabel('half width')
-plt.ylabel('peak to through')
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-plt.legend(bbox_to_anchor=(0,1.02,1,0.2), loc="lower left",
-                mode="expand", borderaxespad=0, ncol=2)
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-plt.savefig(output_path / "figures" / "clusters.svg", bbox_inches="tight")
-plt.savefig(output_path / "figures" / "clusters.png", dpi=600, bbox_inches="tight")
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plt.figure(figsize=figsize_gen)
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-size = 5
-mew = .5
-marker_bs = '.'
-marker_ns = '+'
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-plt.scatter(
-    results.query('bs==0')['half_width'], 
-    results.query('bs==0')['peak_to_trough'], 
-    c=color_ns, s=size, marker=marker_ns, linewidth=mew, label='NS')
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-plt.scatter(
-    results.query('bs==1')['half_width'], 
-    results.query('bs==1')['peak_to_trough'], 
-    c=color_bs, s=size, marker=marker_bs, linewidth=mew, label='BS')
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-plt.scatter(
-    results.query('bs==1 and average_firing_rate > 10')['half_width'], 
-    results.query('bs==1 and average_firing_rate > 10')['peak_to_trough'], 
-    c='red', s=size, marker=marker_bs, linewidth=mew, label='BS rate > 10 Hz')
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-plt.xlabel('half width')
-plt.ylabel('peak to through')
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-plt.legend(bbox_to_anchor=(0,1.02,1,0.2), loc="lower left",
-                mode="expand", borderaxespad=0, ncol=2)
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-plt.savefig(output_path / "figures" / "clusters_and_rate.svg", bbox_inches="tight")
-plt.savefig(output_path / "figures" / "clusters_and_rate.png", dpi=600, bbox_inches="tight")
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stim = results.query('stimulated').loc[:, ['half_width', 'peak_to_trough']].dropna()
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-idxs_stim = cluster_waveform_features(stim.half_width, stim.peak_to_trough)
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-results.loc[stim.index, 'bs_stim'] = idxs_stim
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control = results.query('not stimulated').loc[:, ['half_width', 'peak_to_trough']].dropna()
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-idxs_control = cluster_waveform_features(control.half_width, control.peak_to_trough)
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-results.loc[control.index, 'bs_ctrl'] = idxs_control
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plt.figure(figsize=figsize_gen)
-size = 5
-mew = .5
-marker_bs = '.'
-marker_ns = '+'
-plt.scatter(
-    results.query('bs_stim==0')['half_width'], 
-    results.query('bs_stim==0')['peak_to_trough'], 
-    c=color_stimulated, s=size, marker=marker_ns, 
-    linewidth=mew, label='Stimulated NS', alpha=.5)
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-plt.scatter(
-    results.query('bs_stim==1')['half_width'], 
-    results.query('bs_stim==1')['peak_to_trough'], 
-    c=color_stimulated, s=size, marker=marker_bs, 
-    linewidth=mew, label='Stimulated BS', alpha=.5)
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-plt.scatter(
-    results.query('bs_ctrl==0')['half_width'], 
-    results.query('bs_ctrl==0')['peak_to_trough'], 
-    c=color_control, s=size, marker=marker_ns, 
-    linewidth=mew, label='Control NS', alpha=.5)
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-plt.scatter(
-    results.query('bs_ctrl==1')['half_width'], 
-    results.query('bs_ctrl==1')['peak_to_trough'], 
-    c=color_control, s=size, marker=marker_bs, 
-    linewidth=mew, label='Control BS', alpha=.5)
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-plt.xlabel('half width')
-plt.ylabel('peak to through')
-plt.legend(bbox_to_anchor=(0,1.02,1,0.2), loc="lower left",
-                mode="expand", borderaxespad=0, ncol=2)
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-plt.savefig(output_path / "figures" / "compare-clusters.svg", bbox_inches="tight")
-plt.savefig(output_path / "figures" / "compare-clusters.png", dpi=600, bbox_inches="tight")
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results.average_firing_rate = results.apply(lambda x: float(x.average_firing_rate), axis=1)
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results.frequency = results.apply(
-    lambda x: 
-        float(x.frequency.replace('Hz', '')) if isinstance(x.frequency, str) 
-        else float(x.frequency), axis=1)
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bins=100
-density=True
-cumulative=True
-histtype='step'
-lw = 2
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-plt.figure(figsize=figsize_gen)
-plt.title('Narrow spiking')
-_, bins, _ = plt.hist(
-    results.query('bs_ctrl==0')['average_firing_rate'], 
-    bins=bins, density=density, cumulative=cumulative, 
-    histtype=histtype, color=color_control, lw=lw, label='Control');
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-_, bins, _ = plt.hist(
-    results.query('bs_stim==0')['average_firing_rate'], 
-    bins=bins, density=density, cumulative=cumulative, 
-    histtype=histtype, color=color_stimulated, lw=lw, label='Stimulated');
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-plt.xlim(-.5, 56)
-# plt.legend(bbox_to_anchor=(0,1.02,1,0.2), loc="lower left",
-#                 mode="expand", borderaxespad=0, ncol=2)
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-plt.savefig(output_path / "figures" / "cumulative_ns.svg", bbox_inches="tight")
-plt.savefig(output_path / "figures" / "cumulative_ns.png", dpi=600, bbox_inches="tight")
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bins=100
-density=True
-cumulative=True
-histtype='step'
-lw = 2
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-plt.figure(figsize=figsize_gen)
-plt.title('Narrow spiking')
-_, bins, _ = plt.hist(
-    results.query('bs_ctrl==0')['average_firing_rate'], 
-    bins=bins, density=density, cumulative=cumulative, 
-    histtype=histtype, color=color_control, lw=lw, label='Control');
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-_, bins, _ = plt.hist(
-    results.query('bs_stim==0 and frequency==30')['average_firing_rate'], 
-    bins=bins, density=density, cumulative=cumulative, 
-    histtype=histtype, color=color_stimulated, lw=lw, label='Stimulated');
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-plt.xlim(-.5, 56)
-# plt.legend(bbox_to_anchor=(0,1.02,1,0.2), loc="lower left",
-#                 mode="expand", borderaxespad=0, ncol=2)
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-plt.savefig(output_path / "figures" / "cumulative_ns_30.svg", bbox_inches="tight")
-plt.savefig(output_path / "figures" / "cumulative_ns_30.png", dpi=600, bbox_inches="tight")
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bins=100
-density=True
-cumulative=True
-histtype='step'
-lw = 2
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-plt.figure(figsize=figsize_gen)
-plt.title('Narrow spiking')
-_, bins, _ = plt.hist(
-    results.query('bs_ctrl==0')['average_firing_rate'], 
-    bins=bins, density=density, cumulative=cumulative, 
-    histtype=histtype, color=color_control, lw=lw, label='Control');
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-_, bins, _ = plt.hist(
-    results.query('bs_stim==0 and frequency==11')['average_firing_rate'], 
-    bins=bins, density=density, cumulative=cumulative, 
-    histtype=histtype, color=color_stimulated, lw=lw, label='Stimulated');
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-plt.xlim(-.5, 56)
-# plt.legend(bbox_to_anchor=(0,1.02,1,0.2), loc="lower left",
-#                 mode="expand", borderaxespad=0, ncol=2)
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-plt.savefig(output_path / "figures" / "cumulative_ns_11.svg", bbox_inches="tight")
-plt.savefig(output_path / "figures" / "cumulative_ns_11.png", dpi=600, bbox_inches="tight")
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bins = 100
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-plt.figure(figsize=figsize_gen)
-plt.title('Broad spiking')
-_, bins, _ = plt.hist(
-    results.query('bs_ctrl==1')['average_firing_rate'], 
-    bins=bins, density=density, cumulative=cumulative, 
-    histtype=histtype, color=color_control, lw=lw);
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-_, bins, _ = plt.hist(
-    results.query('bs_stim==1')['average_firing_rate'], 
-    bins=bins, density=density, cumulative=cumulative, 
-    histtype=histtype, color=color_stimulated, lw=lw);
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-plt.xlim(-.5, 44)
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-plt.savefig(output_path / "figures" / "cumulative_bs.svg", bbox_inches="tight")
-plt.savefig(output_path / "figures" / "cumulative_bs.png", dpi=600, bbox_inches="tight")
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bins = 100
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-plt.figure(figsize=figsize_gen)
-plt.title('Broad spiking')
-_, bins, _ = plt.hist(
-    results.query('bs_ctrl==1')['average_firing_rate'], 
-    bins=bins, density=density, cumulative=cumulative, 
-    histtype=histtype, color=color_control, lw=lw);
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-_, bins, _ = plt.hist(
-    results.query('bs_stim==1 and frequency==11')['average_firing_rate'], 
-    bins=bins, density=density, cumulative=cumulative, 
-    histtype=histtype, color=color_stimulated, lw=lw);
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-plt.xlim(-.5, 44)
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-plt.savefig(output_path / "figures" / "cumulative_bs_11.svg", bbox_inches="tight")
-plt.savefig(output_path / "figures" / "cumulative_bs_11.png", dpi=600, bbox_inches="tight")
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bins = 100
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-plt.figure(figsize=figsize_gen)
-plt.title('Broad spiking')
-_, bins, _ = plt.hist(
-    results.query('bs_ctrl==1')['average_firing_rate'], 
-    bins=bins, density=density, cumulative=cumulative, 
-    histtype=histtype, color=color_control, lw=lw);
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-_, bins, _ = plt.hist(
-    results.query('bs_stim==1 and frequency==30')['average_firing_rate'], 
-    bins=bins, density=density, cumulative=cumulative, 
-    histtype=histtype, color=color_stimulated, lw=lw);
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-plt.xlim(-.5, 44)
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-plt.savefig(output_path / "figures" / "cumulative_bs_30.svg", bbox_inches="tight")
-plt.savefig(output_path / "figures" / "cumulative_bs_30.png", dpi=600, bbox_inches="tight")
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plt.figure(figsize=figsize_violin)
-# test = 'permutation_resampling'
-test = 'mann_whitney'
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-plt.title('Broad spiking')
-violinplot(
-    results.query('bs_ctrl==1')['average_firing_rate'].to_numpy(), 
-    results.query('bs_stim==1')['average_firing_rate'].to_numpy(), 
-    test=test)
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-plt.savefig(output_path / "figures" / "rates_bs.svg", bbox_inches="tight")
-plt.savefig(output_path / "figures" / "rates_bs.png", dpi=600, bbox_inches="tight")
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-plt.figure(figsize=figsize_violin)
-plt.title('Narrow spiking')
-violinplot(
-    results.query('bs_ctrl==0')['average_firing_rate'].to_numpy(), 
-    results.query('bs_stim==0')['average_firing_rate'].to_numpy(), 
-    test=test)
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-plt.savefig(output_path / "figures" / "rates_ns.svg", bbox_inches="tight")
-plt.savefig(output_path / "figures" / "rates_ns.png", dpi=600, bbox_inches="tight")
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U-test: U value 104995.0 p value 0.047251399227811186
-U-test: U value 13472.0 p value 0.6449484150738996
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plt.figure(figsize=figsize_violin)
-# test = 'permutation_resampling'
-test = 'mann_whitney'
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-plt.title('Broad spiking')
-violinplot(
-    results.query('bs_ctrl==1')['average_firing_rate'].to_numpy(), 
-    results.query('bs_stim==1 and frequency==11')['average_firing_rate'].to_numpy(), 
-    test=test)
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-plt.savefig(output_path / "figures" / "rates_bs_11.svg", bbox_inches="tight")
-plt.savefig(output_path / "figures" / "rates_bs_11.png", dpi=600, bbox_inches="tight")
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-plt.figure(figsize=figsize_violin)
-plt.title('Narrow spiking')
-violinplot(
-    results.query('bs_ctrl==0')['average_firing_rate'].to_numpy(), 
-    results.query('bs_stim==0 and frequency==11')['average_firing_rate'].to_numpy(), 
-    test=test)
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-plt.savefig(output_path / "figures" / "rates_ns_11.svg", bbox_inches="tight")
-plt.savefig(output_path / "figures" / "rates_ns_11.png", dpi=600, bbox_inches="tight")
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U-test: U value 63173.0 p value 0.3220377333457417
-U-test: U value 8043.0 p value 0.32905688838219194
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plt.figure(figsize=figsize_violin)
-# test = 'permutation_resampling'
-test = 'mann_whitney'
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-plt.title('Broad spiking')
-violinplot(
-    results.query('bs_ctrl==1')['average_firing_rate'].to_numpy(), 
-    results.query('bs_stim==1 and frequency==30')['average_firing_rate'].to_numpy(), 
-    test=test)
-
-plt.savefig(output_path / "figures" / "rates_bs.svg", bbox_inches="tight")
-plt.savefig(output_path / "figures" / "rates_bs.png", dpi=600, bbox_inches="tight")
-
-plt.figure(figsize=figsize_violin)
-plt.title('Narrow spiking')
-violinplot(
-    results.query('bs_ctrl==0')['average_firing_rate'].to_numpy(), 
-    results.query('bs_stim==0 and frequency==30')['average_firing_rate'].to_numpy(), 
-    test=test)
-
-plt.savefig(output_path / "figures" / "rates_ns_30.svg", bbox_inches="tight")
-plt.savefig(output_path / "figures" / "rates_ns_30.png", dpi=600, bbox_inches="tight")
-
- -
-
-
- -
-
- - -
- -
- - -
-
U-test: U value 41822.0 p value 0.016842133424593897
-U-test: U value 5429.0 p value 0.7318233387867226
-
-
-
- -
- -
- - - - -
- -
- -
- -
- -
- - - - -
- -
- -
- -
-
- -
-
-
-
In [101]:
-
-
-
columns = [
-    'average_firing_rate',
-    'half_width',
-    'peak_to_trough'
-]
-
-
-def summarize(data):
-    return "{:.2f} ± {:.2f} ({})".format(data.mean(), data.sem(), sum(~np.isnan(data)))
-
-
-bs = pd.DataFrame()
-
-bs['Control'] = results.query('bs_ctrl==1')[columns].agg(summarize)
-bs['Stimulated'] = results.query('bs_stim==1')[columns].agg(summarize)
-
-ns = pd.DataFrame()
-
-ns['Control'] = results.query('bs_ctrl==0')[columns].agg(summarize)
-ns['Stimulated'] = results.query('bs_stim==0')[columns].agg(summarize)
-
-
-def MWU(column, df, cluster, extra):
-    '''
-    Mann Whitney U
-    '''
-    Uvalue, pvalue = scipy.stats.mannwhitneyu(
-        df.query('bs_ctrl=={} {}'.format(cluster, extra))[column].dropna(), 
-        df.query('bs_stim=={} {}'.format(cluster, extra))[column].dropna(),
-        alternative='two-sided')
-
-    return "{:.2f}, {:.3f}".format(Uvalue, pvalue)
-
-
-def PRS(column, df, cluster, extra):
-    '''
-    Permutation ReSampling
-    '''
-    pvalue, observed_diff, diffs = permutation_resampling(
-        df.query('bs_ctrl=={} {}'.format(cluster, extra))[column].dropna(), 
-        df.query('bs_stim=={} {}'.format(cluster, extra))[column].dropna())
-
-    return "{:.2f}, {:.3f}".format(observed_diff, pvalue)
-
-
-bs['MWU'] = list(map(lambda x: MWU(x, results, 1, ''), columns))
-bs['PRS'] = list(map(lambda x: PRS(x, results, 1, ''), columns))
-
-ns['MWU'] = list(map(lambda x: MWU(x, results, 0, ''), columns))
-ns['PRS'] = list(map(lambda x: PRS(x, results, 0, ''), columns))
-
-bs.to_latex(output_path / "statistics" / "broad_spiking.tex")
-bs.to_csv(output_path / "statistics" / "broad_spiking.csv")
-
-ns.to_latex(output_path / "statistics" / "narrow_spiking.tex")
-ns.to_csv(output_path / "statistics" / "narrow_spiking.csv")
-
- -
-
-
- -
-
-
-
In [99]:
-
-
-
columns = [
-    'average_firing_rate',
-    'half_width',
-    'peak_to_trough'
-]
-
-
-def summarize(data):
-    return "{:.2f} ± {:.2f} ({})".format(data.mean(), data.sem(), sum(~np.isnan(data)))
-
-
-bs = pd.DataFrame()
-
-bs['Control'] = results.query('bs_ctrl==1')[columns].agg(summarize)
-bs['Stimulated'] = results.query('bs_stim==1 and frequency==11')[columns].agg(summarize)
-
-ns = pd.DataFrame()
-
-ns['Control'] = results.query('bs_ctrl==0')[columns].agg(summarize)
-ns['Stimulated'] = results.query('bs_stim==0 and frequency==11')[columns].agg(summarize)
-
-
-def MWU(column, df, cluster, extra):
-    '''
-    Mann Whitney U
-    '''
-    Uvalue, pvalue = scipy.stats.mannwhitneyu(
-        df.query('bs_ctrl=={} {}'.format(cluster, extra))[column].dropna(), 
-        df.query('bs_stim=={} {} and frequency==11'.format(cluster, extra))[column].dropna(),
-        alternative='two-sided')
-
-    return "{:.2f}, {:.3f}".format(Uvalue, pvalue)
-
-
-def PRS(column, df, cluster, extra):
-    '''
-    Permutation ReSampling
-    '''
-    pvalue, observed_diff, diffs = permutation_resampling(
-        df.query('bs_ctrl=={} {}'.format(cluster, extra))[column].dropna(), 
-        df.query('bs_stim=={} {} and frequency==11'.format(cluster, extra))[column].dropna())
-
-    return "{:.2f}, {:.3f}".format(observed_diff, pvalue)
-
-
-bs['MWU'] = list(map(lambda x: MWU(x, results, 1, ''), columns))
-bs['PRS'] = list(map(lambda x: PRS(x, results, 1, ''), columns))
-
-ns['MWU'] = list(map(lambda x: MWU(x, results, 0, ''), columns))
-ns['PRS'] = list(map(lambda x: PRS(x, results, 0, ''), columns))
-
-bs.to_latex(output_path / "statistics" / "broad_spiking_11.tex")
-bs.to_csv(output_path / "statistics" / "broad_spiking_11.csv")
-
-ns.to_latex(output_path / "statistics" / "narrow_spiking_11.tex")
-ns.to_csv(output_path / "statistics" / "narrow_spiking_11.csv")
-
- -
-
-
- -
-
-
-
In [100]:
-
-
-
columns = [
-    'average_firing_rate',
-    'half_width',
-    'peak_to_trough'
-]
-
-
-def summarize(data):
-    return "{:.2f} ± {:.2f} ({})".format(data.mean(), data.sem(), sum(~np.isnan(data)))
-
-
-bs = pd.DataFrame()
-
-bs['Control'] = results.query('bs_ctrl==1')[columns].agg(summarize)
-bs['Stimulated'] = results.query('bs_stim==1 and frequency==30')[columns].agg(summarize)
-
-ns = pd.DataFrame()
-
-ns['Control'] = results.query('bs_ctrl==0')[columns].agg(summarize)
-ns['Stimulated'] = results.query('bs_stim==0 and frequency==30')[columns].agg(summarize)
-
-
-def MWU(column, df, cluster, extra):
-    '''
-    Mann Whitney U
-    '''
-    Uvalue, pvalue = scipy.stats.mannwhitneyu(
-        df.query('bs_ctrl=={} {}'.format(cluster, extra))[column].dropna(), 
-        df.query('bs_stim=={} {} and frequency==30'.format(cluster, extra))[column].dropna(),
-        alternative='two-sided')
-
-    return "{:.2f}, {:.3f}".format(Uvalue, pvalue)
-
-
-def PRS(column, df, cluster, extra):
-    '''
-    Permutation ReSampling
-    '''
-    pvalue, observed_diff, diffs = permutation_resampling(
-        df.query('bs_ctrl=={} {}'.format(cluster, extra))[column].dropna(), 
-        df.query('bs_stim=={} {} and frequency==30'.format(cluster, extra))[column].dropna())
-
-    return "{:.2f}, {:.3f}".format(observed_diff, pvalue)
-
-
-bs['MWU'] = list(map(lambda x: MWU(x, results, 1, ''), columns))
-bs['PRS'] = list(map(lambda x: PRS(x, results, 1, ''), columns))
-
-ns['MWU'] = list(map(lambda x: MWU(x, results, 0, ''), columns))
-ns['PRS'] = list(map(lambda x: PRS(x, results, 0, ''), columns))
-
-bs.to_latex(output_path / "statistics" / "broad_spiking_30.tex")
-bs.to_csv(output_path / "statistics" / "broad_spiking_30.csv")
-
-ns.to_latex(output_path / "statistics" / "narrow_spiking_30.tex")
-ns.to_csv(output_path / "statistics" / "narrow_spiking_30.csv")
-
- -
-
-
- -
-
-
-
In [97]:
-
-
-
bs
-
- -
-
-
- -
-
- - -
- -
Out[97]:
- - - -
-
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
ControlStimulatedMWUPRS
average_firing_rate9.53 ± 0.37 (491)10.62 ± 0.44 (462)104995.00, 0.0471.09, 0.058
half_width0.30 ± 0.00 (491)0.30 ± 0.00 (462)116089.00, 0.5300.00, 0.420
peak_to_trough0.66 ± 0.00 (491)0.67 ± 0.01 (462)116944.00, 0.4070.00, 0.522
-
-
- -
- -
-
- -
-
-
-
In [98]:
-
-
-
ns
-
- -
-
-
- -
-
- - -
- -
Out[98]:
- - - -
-
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
ControlStimulatedMWUPRS
average_firing_rate21.22 ± 1.17 (147)20.20 ± 0.96 (178)13472.00, 0.6451.02, 0.501
half_width0.23 ± 0.01 (147)0.23 ± 0.00 (178)12839.00, 0.7730.01, 0.454
peak_to_trough0.29 ± 0.00 (147)0.29 ± 0.00 (178)13194.50, 0.8950.00, 0.893
-
-
- -
- -
-
- -
-
-
-
-

example waveforms

-
-
-
-
-
-
In [102]:
-
-
-
def normalize(a):
-    t = a - a.min()
-    return t / t.max()
-
- -
-
-
- -
-
-
-
In [ ]:
-
-
-
'half_width','peak_to_trough'
-
- -
-
-
- -
-
-
-
In [142]:
-
-
-
plt.figure(figsize=figsize_gen)
-
-
-lw = 3
-
-row = results.query('bs==1').sort_values('half_width', ascending=False).iloc[50]
-template = data_loader.template(
-    row.action, row.channel_group, row.unit_name)
-
-mean_wf = template.data
-peak_wf = mean_wf[np.argmin(mean_wf.min(1))]
-plt.plot(normalize(peak_wf.T), color=color_bs, lw=lw)
-
-
-row = results.query('bs==0').sort_values('half_width').iloc[10]
-template = data_loader.template(
-    row.action, row.channel_group, row.unit_name)
-
-mean_wf = template.data
-peak_wf = mean_wf[np.argmin(mean_wf.min(1))]
-plt.plot(normalize(peak_wf.T), color=color_ns, lw=lw)
-
-# plt.savefig(output_path / "figures" / "example_waveforms.svg", bbox_inches="tight")
-# plt.savefig(output_path / "figures" / "example_waveforms.png", dpi=600, bbox_inches="tight")
-
- -
-
-
- -
-
- - -
- -
Out[142]:
- - - - -
-
[<matplotlib.lines.Line2D at 0x7f05ec0bc7f0>]
-
- -
- -
- -
- - - - -
- -
- -
- -
-
- -
-
-
-
-

Store results in Expipe action

-
-
-
-
-
-
In [105]:
-
-
-
action = project.require_action("waveform-analysis")
-
- -
-
-
- -
-
-
-
In [106]:
-
-
-
stuff = {
-    "figures": "figures",
-    "statistics": "statistics"
-}
-
-for key, value in stuff.items():
-    action.data[key] = value
-    data_path = action.data_path(key)
-    data_path.parent.mkdir(exist_ok=True, parents=True)
-    source = output_path / value
-    if source.is_file():
-        shutil.copy(source, data_path)
-    else:
-        copy_tree(str(source), str(data_path))
-
- -
-
-
- -
-
-
-
In [108]:
-
-
-
septum_mec.analysis.registration.store_notebook(action, "10_waveform_analysis.ipynb")
-
- -
-
-
- -
-
-
- - - - - - + + + + +10_waveform_analysis + + + + + + + + + + + + + + + + + + + + + + + +
+
+ +
+
+
In [1]:
+
+
+
%load_ext autoreload
+%autoreload 2
+
+ +
+
+
+ +
+
+
+
In [2]:
+
+
+
import os
+import expipe
+import pathlib
+import numpy as np
+import spatial_maps.stats as stats
+import septum_mec
+import septum_mec.analysis.data_processing as dp
+import septum_mec.analysis.registration
+import head_direction.head as head
+import spatial_maps as sp
+import speed_cells.speed as spd
+import re
+import joblib
+import multiprocessing
+import shutil
+import psutil
+import pandas as pd
+import matplotlib.pyplot as plt
+from distutils.dir_util import copy_tree
+from neo import SpikeTrain
+import scipy
+
+from tqdm import tqdm_notebook as tqdm
+from tqdm._tqdm_notebook import tqdm_notebook
+tqdm_notebook.pandas()
+
+from spike_statistics.core import permutation_resampling
+
+from spikewaveform.core import calculate_waveform_features_from_template, cluster_waveform_features
+
+from septum_mec.analysis.plotting import violinplot
+
+ +
+
+
+ +
+
+ + +
+ +
+ + +
+
14:00:33 [I] klustakwik KlustaKwik2 version 0.2.6
+
+
+
+ +
+
+ +
+
+
+
In [3]:
+
+
+
%matplotlib inline
+color_control = '#4393c3'
+color_stimulated = '#d6604d'
+
+color_bs = '#5aae61'
+color_ns = '#9970ab'
+
+figsize_violin = (1.7, 3)
+figsize_gen = (4, 3)
+
+output_path = pathlib.Path("output") / "waveform-analysis"
+(output_path / "statistics").mkdir(exist_ok=True, parents=True)
+(output_path / "figures").mkdir(exist_ok=True, parents=True)
+output_path.mkdir(exist_ok=True)
+
+ +
+
+
+ +
+
+
+
In [4]:
+
+
+
data_loader = dp.Data()
+actions = data_loader.actions
+project = data_loader.project
+
+ +
+
+
+ +
+
+
+
In [ ]:
+
+
+
identify_neurons = actions['identify-neurons']
+sessions = pd.read_csv(identify_neurons.data_path('sessions'))
+units = pd.read_csv(identify_neurons.data_path('all_non_identified_units'))
+# units = pd.read_csv(identify_neurons.data_path('units'))
+session_units = pd.merge(sessions, units, on='action')
+#########################3
+# session_units = session_units.drop_duplicates('unit_id')
+#################################
+print('N cells:',session_units.shape[0])
+
+ +
+
+
+ +
+
+ + +
+ +
+ + +
+
N cells: 1298
+
+
+
+ +
+
+ +
+
+
+
In [ ]:
+
+
+
session_units.head()
+
+ +
+
+
+ +
+
+ + +
+ +
Out[ ]:
+ + + +
+
+ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
actionbaselineentityfrequencyiiisessionstim_locationstimulatedtagchannel_groupunit_name
01849-060319-3True1849NaNFalseTrue3NaNFalsebaseline ii1104
11849-060319-3True1849NaNFalseTrue3NaNFalsebaseline ii1108
21849-060319-3True1849NaNFalseTrue3NaNFalsebaseline ii185
31849-060319-3True1849NaNFalseTrue3NaNFalsebaseline ii194
41849-060319-3True1849NaNFalseTrue3NaNFalsebaseline ii198
+
+
+ +
+ +
+
+ +
+
+
+
In [ ]:
+
+
+
session_units.groupby('action').count().unit_name.hist()
+# session_units.groupby('action').count().sort_values('unit_name', ascending=False)
+
+ +
+
+
+ +
+
+ + +
+ +
Out[ ]:
+ + + + +
+
action
+1849-110319-2    13
+1849-010319-4    13
+1849-220319-4    13
+1834-220319-3    13
+1833-200619-1    13
+1849-220319-3    13
+1849-060319-3    13
+1834-110319-1    13
+1833-050619-3    13
+1833-120619-3    13
+1833-050619-4    13
+1833-060619-1    13
+1834-120319-3    12
+1834-120319-2    12
+1839-120619-3    12
+1849-220319-5    12
+1834-150319-1    12
+1839-290519-1    12
+1834-010319-5    11
+1849-060319-4    11
+1849-060319-2    11
+1834-120319-4    10
+1839-120619-2    10
+1839-060619-5     9
+1833-050619-1     9
+1834-110319-5     9
+1834-110319-3     9
+1833-120619-4     9
+1834-120319-1     8
+1833-120619-1     7
+1839-290519-3     6
+1839-200619-1     5
+1834-110319-6     5
+1849-110319-3     4
+1839-060619-4     4
+1839-060619-3     4
+1839-120619-1     3
+1839-060619-1     2
+Name: unit_name, dtype: int64
+
+ +
+ +
+
+ +
+
+
+
+

Process all data

+
+
+
+
+
+
In [ ]:
+
+
+
def features(row):
+    action_id = row['action']
+    channel_id = row['channel_group']
+    unit = row['unit_name']
+    template = data_loader.template(action_id, channel_id, unit)
+    spike_times = data_loader.spike_train(action_id, channel_id, unit)
+    half_widths, peak_to_troughs = calculate_waveform_features_from_template(
+        template.data, template.sampling_rate)
+    peak_amps = template.data.min(axis=1)
+    half_widths = half_widths * 1000 # to ms
+    peak_to_troughs = peak_to_troughs * 1000 # to ms
+    idxs = np.argsort(peak_amps)
+    peak_to_trough = np.nan
+    for p2t in peak_to_troughs[idxs]:
+        if np.isfinite(p2t) and p2t > .1:
+            peak_to_trough = p2t
+            break
+    half_width = np.nan
+    for hw in half_widths[idxs]:
+        if np.isfinite(hw):
+            half_width = hw
+            break
+    
+    return pd.Series({
+        'half_width': half_width,
+        'peak_to_trough': peak_to_trough,
+        'average_firing_rate': float(len(spike_times) / spike_times.t_stop),
+        'template': template.data[idxs[0]]
+    })
+
+ +
+
+
+ +
+
+
+
In [ ]:
+
+
+
results = session_units.merge(
+    session_units.progress_apply(features, axis=1), 
+    left_index=True, right_index=True)
+
+ +
+
+
+ +
+
+ + +
+ +
+ + + + + + + +
+
+ + +
+ +
+ +
+
+ +
+
+
+
In [ ]:
+
+
+
%debug
+
+ +
+
+
+ +
+
+
+
In [ ]:
+
+
+
df = results.loc[:, ['half_width', 'peak_to_trough']].dropna()
+
+idxs_df = cluster_waveform_features(df.half_width, df.peak_to_trough)
+
+results.loc[df.index, 'bs'] = idxs_df
+
+ +
+
+
+ +
+
+
+
In [ ]:
+
+
+
plt.figure(figsize=figsize_gen)
+size = 5
+mew = .5
+marker_bs = '.'
+marker_ns = '+'
+
+plt.scatter(
+    results.query('bs==0')['half_width'], 
+    results.query('bs==0')['peak_to_trough'], 
+    c=color_ns, s=size, marker=marker_ns, linewidth=mew, label='NS')
+
+plt.scatter(
+    results.query('bs==1')['half_width'], 
+    results.query('bs==1')['peak_to_trough'], 
+    c=color_bs, s=size, marker=marker_bs, linewidth=mew, label='BS')
+
+plt.xlabel('half width')
+plt.ylabel('peak to through')
+
+plt.legend(bbox_to_anchor=(0,1.02,1,0.2), loc="lower left",
+                mode="expand", borderaxespad=0, ncol=2)
+
+plt.savefig(output_path / "figures" / "clusters.svg", bbox_inches="tight")
+plt.savefig(output_path / "figures" / "clusters.png", dpi=600, bbox_inches="tight")
+
+ +
+
+
+ +
+
+
+
In [ ]:
+
+
+
plt.figure(figsize=figsize_gen)
+
+size = 5
+mew = .5
+marker_bs = '.'
+marker_ns = '+'
+
+plt.scatter(
+    results.query('bs==0')['half_width'], 
+    results.query('bs==0')['peak_to_trough'], 
+    c=color_ns, s=size, marker=marker_ns, linewidth=mew, label='NS')
+
+plt.scatter(
+    results.query('bs==1')['half_width'], 
+    results.query('bs==1')['peak_to_trough'], 
+    c=color_bs, s=size, marker=marker_bs, linewidth=mew, label='BS')
+
+plt.scatter(
+    results.query('bs==1 and average_firing_rate > 10')['half_width'], 
+    results.query('bs==1 and average_firing_rate > 10')['peak_to_trough'], 
+    c='red', s=size, marker=marker_bs, linewidth=mew, label='BS rate > 10 Hz')
+
+plt.xlabel('half width')
+plt.ylabel('peak to through')
+
+plt.legend(bbox_to_anchor=(0,1.02,1,0.2), loc="lower left",
+                mode="expand", borderaxespad=0, ncol=2)
+
+plt.savefig(output_path / "figures" / "clusters_and_rate.svg", bbox_inches="tight")
+plt.savefig(output_path / "figures" / "clusters_and_rate.png", dpi=600, bbox_inches="tight")
+
+ +
+
+
+ +
+
+
+
In [ ]:
+
+
+
stim = results.query('stimulated').loc[:, ['half_width', 'peak_to_trough']].dropna()
+
+idxs_stim = cluster_waveform_features(stim.half_width, stim.peak_to_trough)
+
+results.loc[stim.index, 'bs_stim'] = idxs_stim
+
+ +
+
+
+ +
+
+
+
In [ ]:
+
+
+
control = results.query('not stimulated').loc[:, ['half_width', 'peak_to_trough']].dropna()
+
+idxs_control = cluster_waveform_features(control.half_width, control.peak_to_trough)
+
+results.loc[control.index, 'bs_ctrl'] = idxs_control
+
+ +
+
+
+ +
+
+
+
In [ ]:
+
+
+
plt.figure(figsize=figsize_gen)
+size = 5
+mew = .5
+marker_bs = '.'
+marker_ns = '+'
+plt.scatter(
+    results.query('bs_stim==0')['half_width'], 
+    results.query('bs_stim==0')['peak_to_trough'], 
+    c=color_stimulated, s=size, marker=marker_ns, 
+    linewidth=mew, label='Stimulated NS', alpha=.5)
+
+plt.scatter(
+    results.query('bs_stim==1')['half_width'], 
+    results.query('bs_stim==1')['peak_to_trough'], 
+    c=color_stimulated, s=size, marker=marker_bs, 
+    linewidth=mew, label='Stimulated BS', alpha=.5)
+
+
+plt.scatter(
+    results.query('bs_ctrl==0')['half_width'], 
+    results.query('bs_ctrl==0')['peak_to_trough'], 
+    c=color_control, s=size, marker=marker_ns, 
+    linewidth=mew, label='Control NS', alpha=.5)
+
+plt.scatter(
+    results.query('bs_ctrl==1')['half_width'], 
+    results.query('bs_ctrl==1')['peak_to_trough'], 
+    c=color_control, s=size, marker=marker_bs, 
+    linewidth=mew, label='Control BS', alpha=.5)
+
+plt.xlabel('half width')
+plt.ylabel('peak to through')
+plt.legend(bbox_to_anchor=(0,1.02,1,0.2), loc="lower left",
+                mode="expand", borderaxespad=0, ncol=2)
+
+plt.savefig(output_path / "figures" / "compare-clusters.svg", bbox_inches="tight")
+plt.savefig(output_path / "figures" / "compare-clusters.png", dpi=600, bbox_inches="tight")
+
+ +
+
+
+ +
+
+
+
In [ ]:
+
+
+
results.average_firing_rate = results.apply(lambda x: float(x.average_firing_rate), axis=1)
+
+ +
+
+
+ +
+
+
+
In [ ]:
+
+
+
results.frequency = results.apply(
+    lambda x: 
+        float(x.frequency.replace('Hz', '')) if isinstance(x.frequency, str) 
+        else float(x.frequency), axis=1)
+
+ +
+
+
+ +
+
+
+
In [ ]:
+
+
+
bins=100
+density=True
+cumulative=True
+histtype='step'
+lw = 2
+
+plt.figure(figsize=figsize_gen)
+plt.title('Narrow spiking')
+_, bins, _ = plt.hist(
+    results.query('bs_ctrl==0')['average_firing_rate'], 
+    bins=bins, density=density, cumulative=cumulative, 
+    histtype=histtype, color=color_control, lw=lw, label='Control');
+
+_, bins, _ = plt.hist(
+    results.query('bs_stim==0')['average_firing_rate'], 
+    bins=bins, density=density, cumulative=cumulative, 
+    histtype=histtype, color=color_stimulated, lw=lw, label='Stimulated');
+
+plt.xlim(-.5, 56)
+# plt.legend(bbox_to_anchor=(0,1.02,1,0.2), loc="lower left",
+#                 mode="expand", borderaxespad=0, ncol=2)
+
+plt.savefig(output_path / "figures" / "cumulative_ns.svg", bbox_inches="tight")
+plt.savefig(output_path / "figures" / "cumulative_ns.png", dpi=600, bbox_inches="tight")
+
+ +
+
+
+ +
+
+
+
In [ ]:
+
+
+
bins=100
+density=True
+cumulative=True
+histtype='step'
+lw = 2
+
+plt.figure(figsize=figsize_gen)
+plt.title('Narrow spiking')
+_, bins, _ = plt.hist(
+    results.query('bs_ctrl==0')['average_firing_rate'], 
+    bins=bins, density=density, cumulative=cumulative, 
+    histtype=histtype, color=color_control, lw=lw, label='Control');
+
+_, bins, _ = plt.hist(
+    results.query('bs_stim==0 and frequency==30')['average_firing_rate'], 
+    bins=bins, density=density, cumulative=cumulative, 
+    histtype=histtype, color=color_stimulated, lw=lw, label='Stimulated');
+
+plt.xlim(-.5, 56)
+# plt.legend(bbox_to_anchor=(0,1.02,1,0.2), loc="lower left",
+#                 mode="expand", borderaxespad=0, ncol=2)
+
+plt.savefig(output_path / "figures" / "cumulative_ns_30.svg", bbox_inches="tight")
+plt.savefig(output_path / "figures" / "cumulative_ns_30.png", dpi=600, bbox_inches="tight")
+
+ +
+
+
+ +
+
+
+
In [ ]:
+
+
+
bins=100
+density=True
+cumulative=True
+histtype='step'
+lw = 2
+
+plt.figure(figsize=figsize_gen)
+plt.title('Narrow spiking')
+_, bins, _ = plt.hist(
+    results.query('bs_ctrl==0')['average_firing_rate'], 
+    bins=bins, density=density, cumulative=cumulative, 
+    histtype=histtype, color=color_control, lw=lw, label='Control');
+
+_, bins, _ = plt.hist(
+    results.query('bs_stim==0 and frequency==11')['average_firing_rate'], 
+    bins=bins, density=density, cumulative=cumulative, 
+    histtype=histtype, color=color_stimulated, lw=lw, label='Stimulated');
+
+plt.xlim(-.5, 56)
+# plt.legend(bbox_to_anchor=(0,1.02,1,0.2), loc="lower left",
+#                 mode="expand", borderaxespad=0, ncol=2)
+
+plt.savefig(output_path / "figures" / "cumulative_ns_11.svg", bbox_inches="tight")
+plt.savefig(output_path / "figures" / "cumulative_ns_11.png", dpi=600, bbox_inches="tight")
+
+ +
+
+
+ +
+
+
+
In [ ]:
+
+
+
bins = 100
+
+plt.figure(figsize=figsize_gen)
+plt.title('Broad spiking')
+_, bins, _ = plt.hist(
+    results.query('bs_ctrl==1')['average_firing_rate'], 
+    bins=bins, density=density, cumulative=cumulative, 
+    histtype=histtype, color=color_control, lw=lw);
+
+_, bins, _ = plt.hist(
+    results.query('bs_stim==1')['average_firing_rate'], 
+    bins=bins, density=density, cumulative=cumulative, 
+    histtype=histtype, color=color_stimulated, lw=lw);
+
+plt.xlim(-.5, 44)
+
+plt.savefig(output_path / "figures" / "cumulative_bs.svg", bbox_inches="tight")
+plt.savefig(output_path / "figures" / "cumulative_bs.png", dpi=600, bbox_inches="tight")
+
+ +
+
+
+ +
+
+
+
In [ ]:
+
+
+
bins = 100
+
+plt.figure(figsize=figsize_gen)
+plt.title('Broad spiking')
+_, bins, _ = plt.hist(
+    results.query('bs_ctrl==1')['average_firing_rate'], 
+    bins=bins, density=density, cumulative=cumulative, 
+    histtype=histtype, color=color_control, lw=lw);
+
+_, bins, _ = plt.hist(
+    results.query('bs_stim==1 and frequency==11')['average_firing_rate'], 
+    bins=bins, density=density, cumulative=cumulative, 
+    histtype=histtype, color=color_stimulated, lw=lw);
+
+plt.xlim(-.5, 44)
+
+plt.savefig(output_path / "figures" / "cumulative_bs_11.svg", bbox_inches="tight")
+plt.savefig(output_path / "figures" / "cumulative_bs_11.png", dpi=600, bbox_inches="tight")
+
+ +
+
+
+ +
+
+
+
In [ ]:
+
+
+
bins = 100
+
+plt.figure(figsize=figsize_gen)
+plt.title('Broad spiking')
+_, bins, _ = plt.hist(
+    results.query('bs_ctrl==1')['average_firing_rate'], 
+    bins=bins, density=density, cumulative=cumulative, 
+    histtype=histtype, color=color_control, lw=lw);
+
+_, bins, _ = plt.hist(
+    results.query('bs_stim==1 and frequency==30')['average_firing_rate'], 
+    bins=bins, density=density, cumulative=cumulative, 
+    histtype=histtype, color=color_stimulated, lw=lw);
+
+plt.xlim(-.5, 44)
+
+plt.savefig(output_path / "figures" / "cumulative_bs_30.svg", bbox_inches="tight")
+plt.savefig(output_path / "figures" / "cumulative_bs_30.png", dpi=600, bbox_inches="tight")
+
+ +
+
+
+ +
+
+
+
In [ ]:
+
+
+
plt.figure(figsize=figsize_violin)
+# test = 'permutation_resampling'
+test = 'mann_whitney'
+
+plt.title('Broad spiking')
+violinplot(
+    results.query('bs_ctrl==1')['average_firing_rate'].to_numpy(), 
+    results.query('bs_stim==1')['average_firing_rate'].to_numpy(), 
+    test=test)
+
+plt.savefig(output_path / "figures" / "rates_bs.svg", bbox_inches="tight")
+plt.savefig(output_path / "figures" / "rates_bs.png", dpi=600, bbox_inches="tight")
+
+plt.figure(figsize=figsize_violin)
+plt.title('Narrow spiking')
+violinplot(
+    results.query('bs_ctrl==0')['average_firing_rate'].to_numpy(), 
+    results.query('bs_stim==0')['average_firing_rate'].to_numpy(), 
+    test=test)
+
+plt.savefig(output_path / "figures" / "rates_ns.svg", bbox_inches="tight")
+plt.savefig(output_path / "figures" / "rates_ns.png", dpi=600, bbox_inches="tight")
+
+ +
+
+
+ +
+
+
+
In [ ]:
+
+
+
plt.figure(figsize=figsize_violin)
+# test = 'permutation_resampling'
+test = 'mann_whitney'
+
+plt.title('Broad spiking')
+violinplot(
+    results.query('bs_ctrl==1')['average_firing_rate'].to_numpy(), 
+    results.query('bs_stim==1 and frequency==11')['average_firing_rate'].to_numpy(), 
+    test=test)
+
+plt.savefig(output_path / "figures" / "rates_bs_11.svg", bbox_inches="tight")
+plt.savefig(output_path / "figures" / "rates_bs_11.png", dpi=600, bbox_inches="tight")
+
+plt.figure(figsize=figsize_violin)
+plt.title('Narrow spiking')
+violinplot(
+    results.query('bs_ctrl==0')['average_firing_rate'].to_numpy(), 
+    results.query('bs_stim==0 and frequency==11')['average_firing_rate'].to_numpy(), 
+    test=test)
+
+plt.savefig(output_path / "figures" / "rates_ns_11.svg", bbox_inches="tight")
+plt.savefig(output_path / "figures" / "rates_ns_11.png", dpi=600, bbox_inches="tight")
+
+ +
+
+
+ +
+
+
+
In [ ]:
+
+
+
plt.figure(figsize=figsize_violin)
+# test = 'permutation_resampling'
+test = 'mann_whitney'
+
+plt.title('Broad spiking')
+violinplot(
+    results.query('bs_ctrl==1')['average_firing_rate'].to_numpy(), 
+    results.query('bs_stim==1 and frequency==30')['average_firing_rate'].to_numpy(), 
+    test=test)
+
+plt.savefig(output_path / "figures" / "rates_bs.svg", bbox_inches="tight")
+plt.savefig(output_path / "figures" / "rates_bs.png", dpi=600, bbox_inches="tight")
+
+plt.figure(figsize=figsize_violin)
+plt.title('Narrow spiking')
+violinplot(
+    results.query('bs_ctrl==0')['average_firing_rate'].to_numpy(), 
+    results.query('bs_stim==0 and frequency==30')['average_firing_rate'].to_numpy(), 
+    test=test)
+
+plt.savefig(output_path / "figures" / "rates_ns_30.svg", bbox_inches="tight")
+plt.savefig(output_path / "figures" / "rates_ns_30.png", dpi=600, bbox_inches="tight")
+
+ +
+
+
+ +
+
+
+
In [ ]:
+
+
+
columns = [
+    'average_firing_rate',
+    'half_width',
+    'peak_to_trough'
+]
+
+
+def summarize(data):
+    return "{:.2f} ± {:.2f} ({})".format(data.mean(), data.sem(), sum(~np.isnan(data)))
+
+
+bs = pd.DataFrame()
+
+bs['Control'] = results.query('bs_ctrl==1')[columns].agg(summarize)
+bs['Stimulated'] = results.query('bs_stim==1')[columns].agg(summarize)
+
+ns = pd.DataFrame()
+
+ns['Control'] = results.query('bs_ctrl==0')[columns].agg(summarize)
+ns['Stimulated'] = results.query('bs_stim==0')[columns].agg(summarize)
+
+
+def MWU(column, df, cluster, extra):
+    '''
+    Mann Whitney U
+    '''
+    Uvalue, pvalue = scipy.stats.mannwhitneyu(
+        df.query('bs_ctrl=={} {}'.format(cluster, extra))[column].dropna(), 
+        df.query('bs_stim=={} {}'.format(cluster, extra))[column].dropna(),
+        alternative='two-sided')
+
+    return "{:.2f}, {:.3f}".format(Uvalue, pvalue)
+
+
+def PRS(column, df, cluster, extra):
+    '''
+    Permutation ReSampling
+    '''
+    pvalue, observed_diff, diffs = permutation_resampling(
+        df.query('bs_ctrl=={} {}'.format(cluster, extra))[column].dropna(), 
+        df.query('bs_stim=={} {}'.format(cluster, extra))[column].dropna())
+
+    return "{:.2f}, {:.3f}".format(observed_diff, pvalue)
+
+
+bs['MWU'] = list(map(lambda x: MWU(x, results, 1, ''), columns))
+bs['PRS'] = list(map(lambda x: PRS(x, results, 1, ''), columns))
+
+ns['MWU'] = list(map(lambda x: MWU(x, results, 0, ''), columns))
+ns['PRS'] = list(map(lambda x: PRS(x, results, 0, ''), columns))
+
+bs.to_latex(output_path / "statistics" / "broad_spiking.tex")
+bs.to_csv(output_path / "statistics" / "broad_spiking.csv")
+
+ns.to_latex(output_path / "statistics" / "narrow_spiking.tex")
+ns.to_csv(output_path / "statistics" / "narrow_spiking.csv")
+
+ +
+
+
+ +
+
+
+
In [ ]:
+
+
+
columns = [
+    'average_firing_rate',
+    'half_width',
+    'peak_to_trough'
+]
+
+
+def summarize(data):
+    return "{:.2f} ± {:.2f} ({})".format(data.mean(), data.sem(), sum(~np.isnan(data)))
+
+
+bs = pd.DataFrame()
+
+bs['Control'] = results.query('bs_ctrl==1')[columns].agg(summarize)
+bs['Stimulated'] = results.query('bs_stim==1 and frequency==11')[columns].agg(summarize)
+
+ns = pd.DataFrame()
+
+ns['Control'] = results.query('bs_ctrl==0')[columns].agg(summarize)
+ns['Stimulated'] = results.query('bs_stim==0 and frequency==11')[columns].agg(summarize)
+
+
+def MWU(column, df, cluster, extra):
+    '''
+    Mann Whitney U
+    '''
+    Uvalue, pvalue = scipy.stats.mannwhitneyu(
+        df.query('bs_ctrl=={} {}'.format(cluster, extra))[column].dropna(), 
+        df.query('bs_stim=={} {} and frequency==11'.format(cluster, extra))[column].dropna(),
+        alternative='two-sided')
+
+    return "{:.2f}, {:.3f}".format(Uvalue, pvalue)
+
+
+def PRS(column, df, cluster, extra):
+    '''
+    Permutation ReSampling
+    '''
+    pvalue, observed_diff, diffs = permutation_resampling(
+        df.query('bs_ctrl=={} {}'.format(cluster, extra))[column].dropna(), 
+        df.query('bs_stim=={} {} and frequency==11'.format(cluster, extra))[column].dropna())
+
+    return "{:.2f}, {:.3f}".format(observed_diff, pvalue)
+
+
+bs['MWU'] = list(map(lambda x: MWU(x, results, 1, ''), columns))
+bs['PRS'] = list(map(lambda x: PRS(x, results, 1, ''), columns))
+
+ns['MWU'] = list(map(lambda x: MWU(x, results, 0, ''), columns))
+ns['PRS'] = list(map(lambda x: PRS(x, results, 0, ''), columns))
+
+bs.to_latex(output_path / "statistics" / "broad_spiking_11.tex")
+bs.to_csv(output_path / "statistics" / "broad_spiking_11.csv")
+
+ns.to_latex(output_path / "statistics" / "narrow_spiking_11.tex")
+ns.to_csv(output_path / "statistics" / "narrow_spiking_11.csv")
+
+ +
+
+
+ +
+
+
+
In [ ]:
+
+
+
columns = [
+    'average_firing_rate',
+    'half_width',
+    'peak_to_trough'
+]
+
+
+def summarize(data):
+    return "{:.2f} ± {:.2f} ({})".format(data.mean(), data.sem(), sum(~np.isnan(data)))
+
+
+bs = pd.DataFrame()
+
+bs['Control'] = results.query('bs_ctrl==1')[columns].agg(summarize)
+bs['Stimulated'] = results.query('bs_stim==1 and frequency==30')[columns].agg(summarize)
+
+ns = pd.DataFrame()
+
+ns['Control'] = results.query('bs_ctrl==0')[columns].agg(summarize)
+ns['Stimulated'] = results.query('bs_stim==0 and frequency==30')[columns].agg(summarize)
+
+
+def MWU(column, df, cluster, extra):
+    '''
+    Mann Whitney U
+    '''
+    Uvalue, pvalue = scipy.stats.mannwhitneyu(
+        df.query('bs_ctrl=={} {}'.format(cluster, extra))[column].dropna(), 
+        df.query('bs_stim=={} {} and frequency==30'.format(cluster, extra))[column].dropna(),
+        alternative='two-sided')
+
+    return "{:.2f}, {:.3f}".format(Uvalue, pvalue)
+
+
+def PRS(column, df, cluster, extra):
+    '''
+    Permutation ReSampling
+    '''
+    pvalue, observed_diff, diffs = permutation_resampling(
+        df.query('bs_ctrl=={} {}'.format(cluster, extra))[column].dropna(), 
+        df.query('bs_stim=={} {} and frequency==30'.format(cluster, extra))[column].dropna())
+
+    return "{:.2f}, {:.3f}".format(observed_diff, pvalue)
+
+
+bs['MWU'] = list(map(lambda x: MWU(x, results, 1, ''), columns))
+bs['PRS'] = list(map(lambda x: PRS(x, results, 1, ''), columns))
+
+ns['MWU'] = list(map(lambda x: MWU(x, results, 0, ''), columns))
+ns['PRS'] = list(map(lambda x: PRS(x, results, 0, ''), columns))
+
+bs.to_latex(output_path / "statistics" / "broad_spiking_30.tex")
+bs.to_csv(output_path / "statistics" / "broad_spiking_30.csv")
+
+ns.to_latex(output_path / "statistics" / "narrow_spiking_30.tex")
+ns.to_csv(output_path / "statistics" / "narrow_spiking_30.csv")
+
+ +
+
+
+ +
+
+
+
In [ ]:
+
+
+
bs
+
+ +
+
+
+ +
+
+
+
In [ ]:
+
+
+
ns
+
+ +
+
+
+ +
+
+
+
+

example waveforms

+
+
+
+
+
+
In [ ]:
+
+
+
def normalize(a):
+    t = a - a.min()
+    return t / t.max()
+
+ +
+
+
+ +
+
+
+
In [ ]:
+
+
+
'half_width','peak_to_trough'
+
+ +
+
+
+ +
+
+
+
In [ ]:
+
+
+
plt.figure(figsize=figsize_gen)
+
+
+lw = 3
+
+row = results.query('bs==1').sort_values('half_width', ascending=False).iloc[50]
+template = data_loader.template(
+    row.action, row.channel_group, row.unit_name)
+
+mean_wf = template.data
+peak_wf = mean_wf[np.argmin(mean_wf.min(1))]
+plt.plot(normalize(peak_wf.T), color=color_bs, lw=lw)
+
+
+row = results.query('bs==0').sort_values('half_width').iloc[10]
+template = data_loader.template(
+    row.action, row.channel_group, row.unit_name)
+
+mean_wf = template.data
+peak_wf = mean_wf[np.argmin(mean_wf.min(1))]
+plt.plot(normalize(peak_wf.T), color=color_ns, lw=lw)
+
+plt.savefig(output_path / "figures" / "example_waveforms.svg", bbox_inches="tight")
+plt.savefig(output_path / "figures" / "example_waveforms.png", dpi=600, bbox_inches="tight")
+
+ +
+
+
+ +
+
+
+
+

Store results in Expipe action

+
+
+
+
+
+
In [ ]:
+
+
+
action = project.require_action("waveform-analysis")
+
+ +
+
+
+ +
+
+
+
In [ ]:
+
+
+
action.data['results'] = 'results.csv'
+results.to_csv(action.data_path('results'), index=False)
+
+ +
+
+
+ +
+
+
+
In [ ]:
+
+
+
stuff = {
+    "figures": "figures",
+    "statistics": "statistics"
+}
+
+for key, value in stuff.items():
+    action.data[key] = value
+    data_path = action.data_path(key)
+    data_path.parent.mkdir(exist_ok=True, parents=True)
+    source = output_path / value
+    if source.is_file():
+        shutil.copy(source, data_path)
+    else:
+        copy_tree(str(source), str(data_path))
+
+ +
+
+
+ +
+
+
+
In [ ]:
+
+
+
septum_mec.analysis.registration.store_notebook(action, "10_waveform_analysis.ipynb")
+
+ +
+
+
+ +
+
+
+ + + + + + diff --git a/actions/waveform-analysis/data/10_waveform_analysis.ipynb b/actions/waveform-analysis/data/10_waveform_analysis.ipynb index b50bbefb3..d6c1c6dc7 100644 --- a/actions/waveform-analysis/data/10_waveform_analysis.ipynb +++ b/actions/waveform-analysis/data/10_waveform_analysis.ipynb @@ -1,1507 +1,1189 @@ -{ - "cells": [ - { - "cell_type": "code", - "execution_count": 1, - "metadata": {}, - "outputs": [], - "source": [ - "%load_ext autoreload\n", - "%autoreload 2" - ] - }, - { - "cell_type": "code", - "execution_count": 91, - "metadata": {}, - "outputs": [], - "source": [ - "import os\n", - "import expipe\n", - "import pathlib\n", - "import numpy as np\n", - "import spatial_maps.stats as stats\n", - "import septum_mec\n", - "import septum_mec.analysis.data_processing as dp\n", - "import septum_mec.analysis.registration\n", - "import head_direction.head as head\n", - "import spatial_maps as sp\n", - "import speed_cells.speed as spd\n", - "import re\n", - "import joblib\n", - "import multiprocessing\n", - "import shutil\n", - "import psutil\n", - "import pandas as pd\n", - "import matplotlib.pyplot as plt\n", - "from distutils.dir_util import copy_tree\n", - "from neo import SpikeTrain\n", - "import scipy\n", - "\n", - "from tqdm import tqdm_notebook as tqdm\n", - "from tqdm._tqdm_notebook import tqdm_notebook\n", - "tqdm_notebook.pandas()\n", - "\n", - "from spike_statistics.core import permutation_resampling\n", - "\n", - "from spikewaveform.core import calculate_waveform_features_from_template, cluster_waveform_features\n", - "\n", - "from septum_mec.analysis.plotting import violinplot" - ] - }, - { - "cell_type": "code", - "execution_count": 40, - "metadata": {}, - "outputs": [], - "source": [ - "%matplotlib inline\n", - "color_control = '#4393c3'\n", - "color_stimulated = '#d6604d'\n", - "\n", - "color_bs = '#5aae61'\n", - "color_ns = '#9970ab'\n", - "\n", - "figsize_violin = (1.7, 3)\n", - "figsize_gen = (4, 3)\n", - "\n", - "output_path = pathlib.Path(\"output\") / \"waveform-analysis\"\n", - "(output_path / \"statistics\").mkdir(exist_ok=True, parents=True)\n", - "(output_path / \"figures\").mkdir(exist_ok=True, parents=True)\n", - "output_path.mkdir(exist_ok=True)" - ] - }, - { - "cell_type": "code", - "execution_count": 4, - "metadata": {}, - "outputs": [], - "source": [ - "data_loader = dp.Data()\n", - "actions = data_loader.actions\n", - "project = data_loader.project" - ] - }, - { - "cell_type": "code", - "execution_count": 5, - "metadata": {}, - "outputs": [ - { - "name": "stdout", - "output_type": "stream", - "text": [ - "N cells: 1281\n" - ] - } - ], - "source": [ - "identify_neurons = actions['identify-neurons']\n", - "sessions = pd.read_csv(identify_neurons.data_path('sessions'))\n", - "units = pd.read_csv(identify_neurons.data_path('units'))\n", - "session_units = pd.merge(sessions, units, on='action')\n", - "#########################3\n", - "# session_units = session_units.drop_duplicates('unit_id')\n", - "#################################\n", - "print('N cells:',session_units.shape[0])" - ] - }, - { - "cell_type": "code", - "execution_count": 6, - "metadata": {}, - "outputs": [ - { - "data": { - "text/html": [ - "
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" - ], - "text/plain": [ - " action control entity frequency session stim_location \\\n", - "0 1849-060319-3 True 1849 NaN 3 NaN \n", - "1 1849-060319-3 True 1849 NaN 3 NaN \n", - "2 1849-060319-3 True 1849 NaN 3 NaN \n", - "3 1849-060319-3 True 1849 NaN 3 NaN \n", - "4 1849-060319-3 True 1849 NaN 3 NaN \n", - "\n", - " stimulated tag channel_group unit_name \n", - "0 False baseline ii 1 104 \n", - "1 False baseline ii 1 108 \n", - "2 False baseline ii 1 85 \n", - "3 False baseline ii 1 94 \n", - "4 False baseline ii 1 98 " - ] - }, - "execution_count": 6, - "metadata": {}, - "output_type": "execute_result" - } - ], - "source": [ - "session_units.head()" - ] - }, - { - "cell_type": "code", - "execution_count": 7, - "metadata": {}, - "outputs": [ - { - "data": { - "text/plain": [ - "" - ] - }, - "execution_count": 7, - "metadata": {}, - "output_type": "execute_result" - }, - { - "data": { - "image/png": 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" - ] - }, - "metadata": { - "needs_background": "light" - }, - "output_type": "display_data" - } - ], - "source": [ - "session_units.groupby('action').count().unit_name.hist()" - ] - }, - { - "cell_type": "markdown", - "metadata": {}, - "source": [ - "# Process all data" - ] - }, - { - "cell_type": "code", - "execution_count": 8, - "metadata": {}, - "outputs": [], - "source": [ - "def features(row):\n", - " action_id = row['action']\n", - " channel_id = row['channel_group']\n", - " unit = row['unit_name']\n", - " template = data_loader.template(action_id, channel_id, unit)\n", - " spike_times = data_loader.spike_train(action_id, channel_id, unit)\n", - " half_widths, peak_to_troughs = calculate_waveform_features_from_template(\n", - " template.data, template.sampling_rate)\n", - " peak_amps = template.data.min(axis=1)\n", - " half_widths = half_widths * 1000 # to ms\n", - " peak_to_troughs = peak_to_troughs * 1000 # to ms\n", - " idxs = np.argsort(peak_amps)\n", - " peak_to_trough = np.nan\n", - " for p2t in peak_to_troughs[idxs]:\n", - " if np.isfinite(p2t) and p2t > .1:\n", - " peak_to_trough = p2t\n", - " break\n", - " half_width = np.nan\n", - " for hw in half_widths[idxs]:\n", - " if np.isfinite(hw):\n", - " half_width = hw\n", - " break\n", - " \n", - " return pd.Series({\n", - " 'half_width': half_width,\n", - " 'peak_to_trough': peak_to_trough,\n", - " 'average_firing_rate': float(len(spike_times) / spike_times.t_stop),\n", - " 'template': template.data[idxs[0]]\n", - " })" - ] - }, - { - "cell_type": "code", - "execution_count": 9, - "metadata": {}, - "outputs": [ - { - "data": { - "application/vnd.jupyter.widget-view+json": { - "model_id": "4e27da53954b492ba7a0ab4933847df2", - "version_major": 2, - "version_minor": 0 - }, - "text/plain": [ - "HBox(children=(IntProgress(value=0, max=1281), HTML(value='')))" - ] - }, - "metadata": {}, - "output_type": "display_data" - }, - { - "name": "stdout", - "output_type": "stream", - "text": [ - "\n" - ] - } - ], - "source": [ - "results = session_units.merge(\n", - " session_units.progress_apply(features, axis=1), \n", - " left_index=True, right_index=True)" - ] - }, - { - "cell_type": "code", - "execution_count": 10, - "metadata": {}, - "outputs": [], - "source": [ - "df = results.loc[:, ['half_width', 'peak_to_trough']].dropna()\n", - "\n", - "idxs_df = cluster_waveform_features(df.half_width, df.peak_to_trough)\n", - "\n", - "results.loc[df.index, 'bs'] = idxs_df" - ] - }, - { - "cell_type": "code", - "execution_count": 86, - "metadata": {}, - "outputs": [ - { - "data": { - "image/png": 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\n", - "text/plain": [ - "
" - ] - }, - "metadata": { - "needs_background": "light" - }, - "output_type": "display_data" - } - ], - "source": [ - "plt.figure(figsize=figsize_gen)\n", - "size = 5\n", - "mew = .5\n", - "marker_bs = '.'\n", - "marker_ns = '+'\n", - "\n", - "plt.scatter(\n", - " results.query('bs==0')['half_width'], \n", - " results.query('bs==0')['peak_to_trough'], \n", - " c=color_ns, s=size, marker=marker_ns, linewidth=mew, label='NS')\n", - "\n", - "plt.scatter(\n", - " results.query('bs==1')['half_width'], \n", - " results.query('bs==1')['peak_to_trough'], \n", - " c=color_bs, s=size, marker=marker_bs, linewidth=mew, label='BS')\n", - "\n", - "plt.xlabel('half width')\n", - "plt.ylabel('peak to through')\n", - "\n", - "plt.legend(bbox_to_anchor=(0,1.02,1,0.2), loc=\"lower left\",\n", - " mode=\"expand\", borderaxespad=0, ncol=2)\n", - "\n", - "plt.savefig(output_path / \"figures\" / \"clusters.svg\", bbox_inches=\"tight\")\n", - "plt.savefig(output_path / \"figures\" / \"clusters.png\", dpi=600, bbox_inches=\"tight\")" - ] - }, - { - "cell_type": "code", - "execution_count": 85, - "metadata": {}, - "outputs": [ - { - "data": { - "image/png": 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690Bjk20TQoBgJzJD8WYO9jUJJ3wRmIZKqY4A7r8NlFJNcZX+CzWcYCcyQ/Fmrsw1CUWBDCS+CMwEXMMnZOMKiyYCdwJ/MdMwITQIdq1FsAWuNCpzTUJRIANJhX2RtNbfAd1LLN5gjjlCpOAsspOZNoe2w0ZjjY4pc7twb6kJRYEMJBUKjFIqE1c9jIeTWutu5pkkRAKZaXNofffDZALt73ww2OYAP3ceTIrrSEbeLr90Igx3gawuvly9jkAnIAm4B1hjpkGRiLPIzr4PZuEssvu0vCbQdthoDsybQdtho4NtSjGecObj3Z9GdFjjVwzDqNTLZrP9r7L7mPW67bbbjJrA3oUzjfPRVmPvwpk+Lfc3DqfD2Jqz3XA4HaZ+T3Ux207P8e0Oe1hcj2Bis9k2GD7co76ESC/yc4jUEojMdLiJtB02mkz3X1+W+xvPk/vJ1EdC2p03207vcCaUr0M44cuAU7u83m8FPjXJlojFGh1Tah6irOX+xoxEpBmDIUV6wjQc8eWXn4+r5qUH0Bwo8OXASqmeSqk1pSy/VSn1vVLqG6XUA+5ldZRSaUqpr5RSK5VScb6fglBdzGiKNqN5NthN5kLl8eWXmgW0wzW9SBtcE6qVSzkTr8UAU4EbgWuB3yilmgHjgO1a677Ae8Bzvp9CeOGvxG1ljxPogq9AehuRXswWyvgiMJdrrZ/UWi/TWj8OdPBhn7ImXusE7NVaH9danwe+Bn4B9OHn0OsT4AYfviMkqejGL26eTSvZh7RyVPY4VfEoqiOGgfQ2Ir2YLZTxJQdTWylVV2t91j31a1RFO2it05RSbUpZ1RA44fX5FK7pY72Xe5aFJRXVd/grcVvZ41TFowjFWpXSkNxM6OLL4+UfwFal1FJgC64Qp6qc5MLR8RoA+SWWe5aFJYmD72H3+FEkDr6neJm3J+BJ3JZXveoLlT1OVTyKimpVQiU0CZXcTKhcj1DCl18kG+iJq+9Rb611dQb93glcrpRqopS6BFd49A2wFrjFvc3NuOZNCkuylr+Pbfp8spa/D7jEZedTY2g9aly54Yy3CIVKgV1FIuYJTVbolXJTIaFaafgSIk3WWv8C+KmqX+KZeE1r/aZS6gngM1ziNkdrfUgp9QYw1z3x2nnCeI6kkqFLZtocbNPns3vC3XQqJ5zxDkeAsAlNhqpBpO1aTttL21SrdiQc5vipCAnVSqGiSjybzfalzWZbarPZXrLZbFNsNtsUXyr4AvEKh0peh/28sXfhTMNhP1/uenvBmeLtytvHe50vla2Bqn6t7vG35mw3fr3sAWNrznY/WSaYia+VvL48KuYAy3CFN5oLC++ECqgozPB4LlnL33d5P+4wqqx9vFuPfHHJzXbbS+Y/qpqHKO/pL7mN8MUXgVkGnMHVXcCoYNuIxpfcScltvBOp5TU9e/ZLHHxP8fa+uOTVcdurcmNXVdDKS9RKbiOMqcjFsdlsq2022xybzfai+yUhUhnsmT/dsFsx9syffsFy77CmrG1KbleSQHV89KYqYYsZIVm4dMaMJPzW2RGwaK1Dp099qGOxUvjtOpx3PIA1OgZnkZ2MJ+6n4/T5ZIz/ilo9ekIZSczy+h4FquOjN1XxfswY/yTSx1QJZ8oMkZRSl7ibkvcrpXoppWp5LRNKod0dD6AnjEJN/1dxmJOZNoeOr8/HYuD6a40ia/4M2t3xQKWO7a/6mUp9Z4jUlwSCUCkNqGmU58FoXDkXC/BLr+UGrr5JQgms0TF0emU2mb2uKfY02g4bzf55DgynA4s1inZuz8aDr0NHelNek66/j+fL+lDHl2sSLlXLYUdFMZTNZru6xOd+vsRegXiFWg6mKuxZ8IZxPtpq7FnwRvGyipq2y8uNVCVXU1Gupbz1wciPVPY7fbkmFV1z4UJ8zcGUJyx9bDbbb2w22y7339/YbLaHbDZbui8HDsSrJgjMph83Gi/94SZj048bi5dVdEOUd4NV5UZxOB3G5h83GXsWvFF67U053xeM+pXKfqeIh//xRx1MPtAC1yyOLdyvOGBSAByrsKdkTF+yydez3jAM0ru1whL1c7RaUR+g0nIjnuNB2TU0ZWG1WGnw9Xck3jO+1Cby8nIxwaherex3BiN/JbipSIFsNltLX5QqGK9Q9mBKeiEln7qe9XsWvOEaB/Z8YYVP2fI8ieo2Y8tTXqgMfmum1lofDoTQ1SScRXYMh4Osuf+kzdD72HYknaS4jhc8dT3Nzq1uuYOtz4xmf+ovaTv6sVKTjJ4kq9NwMvXb10sdk7btsNHsdzjA4SjutV0ZAjU8Z7gR7gnuYCNXzAQy0+aQeO9ELFFRZPy0h1fXv+aaZ6epIvPDt4oFoO2w0ey881f0mLGMn9b9t8ywaNvhrXw642kMR1GZoYE1OgZLVBSJ906s9mBWws9IFXE18cXNsdlst9hstqdtNttgX7YP1CtUQyT7+UJj7VuTDfv5wp+nwjhfaKQ/es8FYYwnrNl0c3fj3NlTZR6vtJam0ijuOHm+MCQrX8MxDKtsi1WkVB37rbOje9qSMYAduFcp9arpqhfmZPy0h1lxP5Lx0x6sFivJ8Ul8N/dlbK/PY2//nsWDUXkGp+qy5H9cUqd+mcdrN3wMB+bNoN3wMeV+ryfM8XhNgX7qVtR3yV/DhfoTT3K8yH6uVNsrW2woHk8JKlIgm8221uu9xWazfeuLcgXiFYoejMPpMDZnbzU2Z28tfoptzdlu3Js2xlj/8BDjfJTlIg+mZGLW2wPyHDMcnqIVNR+Hogfj+Q3WvjXZL83t4sFU0oMBYpRSnu0sSI/qcknPzWDq2mmc/WgZOBwU2c9x8t9pTOzxEDHPPkfWvBkkDr6HvQtncrLXlWS++xqGw0FR4dniZu3v5r7M1eMms/GxERQVnmX97Bf4+9p/lPpULM1rCFaJf0XNx6HYXOwpCehx7//5pbk9krpX+IIvV+EDYK1SaiquWQA+MNek8CapyeU8vPonrh43mcy0OXw392V6PvwnDi+dxz82zOR0v95kLX+fxHvG85/Zf+BowTESfz2BPbffUDys5lWjniD9hiu4cuYydj7zIFePm8zYvFbU/eLri/rKVMYlN7u/TTjeXB7Ri46pFXa2hwO+9KaehmuIy47AbODHinZwezwzgK7AOWCs1nqve103XAOJe0gFhgDfAbuBdPfypVrrab6dRuiQteRdrpy1nE0PDqb70PtIxHViLW66GWODqxCu7bDR7C2y0/t0HleNegK9fTcdp/+LXePvIsk9Lkzy55v56toOHLv7Fg50iOXy+rGuQjiLhfZ3PljcfJoU15HHe07AaTiLvZiymlXDtb+NNBWHL+X1pm6ulLLh8lrOAduAImCVD8cdAtTWWvcCngGKE8Na6y1a635a637AdCBNa/0pcCWwwLMuHMUFXOLx/cw/8sb1Tfl433+wRsfQe+wfuLJVd57s9Yhro6gooqJj6DnhBQ7+ez61e/YGwygOPi8bMIK9KYn0/XIv9jX/ZUHz42w6vJnMd18rbsb2eC4ZebvA4WDVG//HtsNby/VoSqsQDodexKHkpQmVozwPJhV4FFC4Zne04Jr4/jMfjls8kZrWer1S6qqSGyil6gGTcc0sANAd6K6U+hLIBR7RWmf7eB4hgzU6htQxz5GnV144GLbDwdmPljGrwX4eyG9DbEx99t7Yk/YDRhBduy47v1uPen0emdf0pfCbtSRtymRL/yu5snkKlswtDPvtu3z/RiI2d/7CO9+x/4M3efwvn6FPxNHxb2+VWytT0nPxp1djlqdRma4B4eql1VgqygLbbLZbfMkWl9jnbZvNdrPX5wM2my26xDaP2Gy2yV6fB9tsthvc70fZbLbFFX1PKLYiGYartUT/a7qxZPsS41zROWNrznZDz3vNsFsx1j002LBbMYrAcIKx7ZFRxt6FM41zp/KNbRNGGNsmjDAKjucZ2yeOMrZNGGGcj7IYet4/L2hVKu37StbYVMZWf7XshMLA3YFuqYqUVqOS+LOrwMoq6FbJCdasWuuiEtuMAoZ7fV4NnHW/Xwr8qQrfG1Q8444YDgdt753I5tu78enV/2Vn3i6uPmzQHgvnO9rY8JvBFLZrw6XvfUDh9k10fH0+evxXJL2+ECuwwxpF7dTetLlnPLvGjyLpzgeLPZfSvITSxqHxFX92EQiFaTsC3eXBE76V1n1DMK+rQPFEakqpVGC790qlVCOgltb6oNfit4Fh7vfXAxtNss009n/4FomjHsbpcLBr/EiGfbCZAU9M5+kX/8u1c1eD06Bo21a6v72Cev/9gpT0HHp8sZP9XdvgdJwHiysGveSK7q4BqrDgdNhZN/sFiuzncBpOVuiVvPLNtIvyEaHQBByOrUjVJRRENZTxpZK3YYnPvX047lKgUCm1DtdUs48rpZ5QSg1yr7cBP5TY5xlgnFJqDfAQrvxPyHNRHYrh5MjpI+R3bMfUp3/Jkfh6WHAlsNI7N+NI6zg+eWU89ql/Z/N1KWS1aUr7bVl0nrGIr/q2xQDObd6AYRjgNOg840NSx7/AJzP/j+W7PiJt13KuaZVKUlzHIJ614CESRbUyWAyj/Lo5pdR/gQG4WpD+DNyktb4yALZVyNChQ40lS5YE1YZtR9KLXeTkpor1777I11nrePyl/7DhN4O46s1/k9ekDs1yz2AFnFYLi4d3pePOHJK352AFfmpchyb5BWzr3Jxdyc0ZmraNrLn/xGk4yT7+I44tm3nnV80wLomh72W9WXfoW3HJw4ya1tSulNqotb6o8aYkvpzpVFxzI63F1R+pZzVtq1F4u8jW6Biuuv8ZGg64jW/++SznL7+cr/u0ZW2fdliBvZc15Js+HRj+4RZStucAkB1fnyb5BTiBlB05GFYL3/zzWQDO9OvN+rwt9J37OQ+vOcGDuQnc22UEQ9Ug8WDCjEjto1RmktddAwOu4rcvcQ38PQ9o614mcOGUGk7DyTvfv0v+R4s5vP8Yty/cjNWA7Ph6ALQ7eJJ2B0+yLSmelIxcAJrlnuZow0v4YtxAmh49ycpfteT23NP0+vUE0m+8kp0jO7AvJZGrZ/4boj7iM/sp0lqdvmAuaO+iu4y8XTXmKVmTiNRcTXn/C2d5vW7EJUazgJkBsCvkuWhIzCI762e/wPGPFvHUS6sZvnAzabdfQUbX1rTIPcOZS6zFF7tLRi7bu7QAXD9A7Mnz9H7nM+Ze34x+PzhYFHuUHZ2a0e2Tjbz41ErUpkz0gFTWT3+ORS1P0SuhB3Z7IXsXzsRZZC9+On68+9OIfEqGAxGbq/GlLdv7ZbPZLqnsPma9glkH490T2uF0GGvfmmycj7YaX8183tg551Vj041XGF+8Nsn4y0d/MHa3vdRwgnHqEqthB+PH+HpGkdVifNGnrbE1Kd5wumtiNndpYZyPshjLf92nuE7GCcamlBbGxh++NRxOh7Fk+xJjyh9uNF587lcXfP/WnO2G3WG/oCYjUms0BPPxWx2MUupB4AkgBldjiB1XK1DEUuQsYlvneBzv/ZMOw0aTnpvBrHp7qPPLrjTctpPME9n0X7UZ56rNXJbYmDZZ+RRaoN55JyfqRNEy9wwAfb/OBFxN0xYgeXs2i0deQVZCLc6+MJLmn62nVsfO1Prd70m57ErSczPovD2bgVM+Z/3rvyPr/aG0Gzb6gjDNO/ErNRpCsPHFXxsP9AM+Ae4HIt7//nj3pyzes4IdXVqwd9Fb7M7dxb1f5NFt1Wa6zPiQDmvT8Qye0C4rHytQ291Y16DAgRM4E+26+FYg87IGWIB1v+jAZfVa8uRL/yXp4Hn6fpVJ417XcWViDzLydvHq+tc4+4teHJg3g2vG/J4OIx4qt+4lUuN+IXTwRWAOu/sENdBarwEamWtS6OKpebn58hu5o9NQOm/Ppu2vJ7Jn8Vu8c308H97ZjW2dm9Phh+Mcia+HFThZ28IZr6vsEZV6XnXNZxrUxgnkxNeh5zsrsQBR33yHFSj84F84i+zU/eJrHr9qHCktu/pcUBexcb8QMvgyXMMJpdQQwHCHS7Em2xSyeIccgzsOxNnBzl6ng85HdpG4/xQ/tGsKhkHyjhzi885w5hILDQvLrzPKjq/H7k4tSMnIw4iO4pVnrgeLhZ0dY5nw/g54t0ogAAAP4UlEQVS6LPqPaxDxe8ZzYN4MrHdeEaCzFYTq44vAjAXaA88CTwITTbUohPEOOYoKz7LrmQc5dnkrbpk0E4vDFRQZVki7vQvXfaaJPXkecOVYSvoQTuBIXF3ifipgvy2OhaOuZOWtnXFeEo0VK0M7DqL7XQOxWqzFU5xUtp+RIAQbXwTmLHAV0BpYwc8DQkUM3lWYSXEdWaFX0nbmQrpMm88HI6/g1UnX0XNdJn2+zmTx7V2JzzlVLC7gEhdnib9WoEXeWdZc247tV7bi8nse4dFGrTiQf5C2TdrQtVkXcDjYl/YWbYeNrvFDD9S0SlfBhS+/5Cxc4vIrXD2k3zPVohDDaThZvusjXvlmGst2ruCtje/y4c4lrBvem4WjrmTv/bfhjIniGre4HGrRiMT9eRytZ8UJbLc1BX6+0J5BdQz33xMDb+LJPo/T7tI2RFujWb7nY6IsUVgt1pAchd8s/F3pWtEMB0Jg8EVg2mut/wAUaK1XEGFJ3vTcDNJ2LcdhOEjTy1l3aD19WvXmvtTRHJ/4IDtPZ3JYtWFHUnOiipw8NvVL2h04SewZ13/sljmngJ9FxZORWXRnN/723K9IuPM+DuQfZOq3rwNcNPvjAfcg4TV9lLayWryqKhSRWpofavgSIkUrpWIBlFINgIh6JCTHJ/FUr0cxMNh/LJOlu1fQs9VV7Dq6m7WH1wNw29ur6bo9my7bs0lPiiMlIw+Agmho6g6VtifFkayPknZ7VzLbx+K8/pfsPLabzsv+xZKWpxiSNLj4+7zHeWl/54Ps+2CWqaO0hUJ44l3L401Va3mkiT408EVgnsPV0bEFsB54zFSLQgyrxUq35ikAWNz/vG/CVg1b8e4DPbEYBht6XEaMtRa602Gu2HCQtln5xWr8n4Fd+GR4NDu6tGBo0m0M6jiA7+ZM4eonJtP0jeepe2mbcuedrmyStzKiEcoFeVUVirIEKxiEgoAHDV/KfW02W7TNZmtts9ksvmwfqFeguwp4l97bHXZjScZy49sfvzf+7z/PGXctud/423M3G3YLxr/vvsY4lp9tTH/+NmPJiKsNe5TFWD51opGWsczYeHjzz6X8XsM7+lrW7+t2lRm+UroUmEsoDCXqb3ztKuDLeDBDcc0KcBxoCIzTWv8nANpXIcEYD8aTE9h7dB9L96zA4nDSeXs2xrXXcY0+RZ8JL2JgsPil0fxw+hA7kuK5M+dSbLGX08GHArmKnnbe48+U94SO6KdmiFETfwtfx4PxJUT6PdBTa52rlGqGq6m6XIEpb14k9/ppuGYeOOVeNBhXX6d/AXWAw8D9WuuzhBjpuRm8+vVUkrYfxtKlBf322bl3yud8ftDO2ze14sTfx2PBwg95O3ny5dUsvrMbjfoOoO19j5Lpw3ixFYUrvoYMoRQiRDqR/Fv4IqfHtNa5AFrrI7gG9K6IMudFctMd18h4njmQTgB/AP6lte4LbAZCsvAjOT6JB4+34akpn9N5ezYdhz3At9fauGHuFyRlHGFxYiGX3TmW/uP+yq7xdzF84WaaN2h20XxE5R2/3OlXpfxfCCN88WBOKaU+wzXoVHegrlJqCoDW+rdl7FPmvEhu7+Zy4E23RzRbaz3Hvc8U92afuN9PrfwpmYvVYqX3/b9lf4Nm3Ny3JweXzKX/l7v55O4+3DD2z/S3RlN/zTraDR8Dr8whs1cf2g8b7fNg3JH8tBNqHr4IzDKv94d8PG5D4ITXZ4dSKto9dUk94J/A34Eo4Aul1IYS+5wihOttrNExdBjxEAD2IUW8cnQnN4x9gasvu9rVpOw1xWtNr8AVhPLwZV6kuVU4bnnzIp0FpnnyK0qp1bhyNZ59Ctx/86vwvQHj8N5cWnaIJyamFhndLuPmmFpA1ZqUBaGmEox5kWzAWqVUlFIqBldotMl7H+Bm4CuTbKs2h/fm8tH0/3F4by4pzZJ5uvdjxJ6KB34ujsv54XiQrRSE4GOWwJQ5L5LWeifwPq6ivS+B97TWO4AXgBFKqbVAL+B1k2zzCwPH/4KWHeKxWqzEnopn5YyvObzXNZC3twAFC+mLI4QCFdbBhDLBqIPxiIdHYDzLjh7KJ+Va2wXbedYHA1/rZQShKvizDkbwomWH+IvE5aPp/wMgNqFxUEXFG+mLI4QCIjBVwFtEPILjvbw0LyfQSHO3EApItVYV2Pblz/POeUKh0kQnVLwZQQgWIjCVZNuXu1m/bBvbvtx9QTK3ZEK3ZYf4Kid5g5kcFgR/IgJTSVKutZE6JOWChO7RQ/kXtRpt+3J3lVqSQqEFShD8heRgqoBHXLxDIe8E7+G9uaxfto3UISmVPnZlwqtgt1QJQkWIB+MnvEOilh3iSR2SQmxC4zJDKCg7FPJVXMTTEUIdEZhq4B0Ged/wnjwNUNzC5K8QyoMkkoVwQASminiHQUcP5V/QXO0RF6C4halk7Yxn3+oIRDiLi3hekYEIjI+U1krkybGsX7aNrxZvKl4+cPwvigXFO2zy3nfg+F9ckCiOJCS8ixxEYHygtBvC44V42Ll2f/E2Hq+lvDAonL2P6iLhne+EuwiLwPhAaSHOvi0/AnAi7zSNm/08MsW+LT8W52S8Bcibkv9pvAv3IoXq1AlFCjXB0xOB8RGPuHy1eBMfTf8f7bu1Ir5NE86cKCD/yKni7TyezNFDruFsvPMsJZPB3gnhSBOZmnDzmE1N8PREYHzEIwY71+4nvk0Tvl68mdwffuJAejYxtX8uJ4pv0wRweTbeeRbPDeURHk9xXmxC44sK90r77ppGTbh5AkG4Xx8ptKsAj2exftm2YvHI/eGnC7axF7oG62ud3IKUay/nu4/T2bl2P/BzKOARltiExhcU5wEVikuwO06aRU07H+FiRGDK4avFm4qFAuDYobJH8Wyd3IID6dkcSM8GXJ7MzrX7aRRXvzgX0+madhfdVBWJhzzphXBGBMaNJww5eiif2ITG7Nvy4wXiAuCwXzg6XFSMFYfdSXybJqRcezktO8Sxftk2Wie3oP+Ya4pblGITGnP0UD7rl22jfbdWxWLhq3iIuAjhiikC48PEa48DI9wfV2qtJyulLMCPwB738m+01s+aYV9JvCtvfSG+TROaJjQuFqB23VoVeyKpQ1JYv2zbBf2EPM3WpQ1IJeIh1GTM8mCKJ15zD/r9Kq7ZG1FKtQNGAT0BJ/C1UmoprtkGNmmtbzXJplIpGQZVhCfP0rJDPO27tQK4KJ9S1sh2lRET6cgo1ATMEpgyJ14DDgL9tdYOAPfMAoW4JnVLUEp9gWvqkse11trfhi2btpra9WtRr1EdDmRkc+Z4QZnbWqxwWVILCk+fo8eAn0eHKytv4q9kbE1O7AqRhVkCU+bEa1prO3DUHRL9Ddistd6tlGoOvKi1XqSU6gPMA672p1HLpq2+qAXIgzXKQqtOzTl2KJ+mCY05kJ7NgHGl3+Cl9SHyZzJWErtCTcEsgSlv4jWUUrWBObhmcHzYvXgDUASgtf5aKdVSKWXRWvtt2oPa9WsVv2/crAGX1HFN59pjQPJFN3NZIYqnQtfsfIqIi1ATMEtg1gK3Ah+WnHjN7bksB1ZrrV/22ud54BjwV6VUV+CgP8Xl8N5c+o+5hk9nr6Veozr0HX5ludv72mwsuRJBKBuzBGYp8Cv3xGsW4H6l1BPAXlzzUV8L1FJK3eze/lngJWCeUmoALk/mPn8Z453T6D/mmmofL5RmDxCEUCZiJl4zy9MQD0aIRHydeC1i+iKZJQIiLoJQNhEjMBVREzsUCkKwEYFBhg4QBLMQgUHqTgTBLERg3Ii4BBfxHmsmIjBC0JEQteYiAiMEHQlRay4iMEJIIOISXMzyHkVgBCHCMTNEFYERhAjHzBBVBEYQBNNCVBEYQRBMQwRGEATTEIERBME0RGAEQTANERhBEEwjrCde27Fjx1GlVFaw7RCECCTRl43CekQ7QRBCGwmRBEEwDREYQRBMQwRGEATTEIERBME0RGAEQTANERhBEEwjrOtgqopSygrMALoC54CxWuu9XusfB0a4P67UWk8OvJXVp6Lz9NrmY2C51npm4K2sHj78ljfjmpbYAmwExvtzSuJA4cN5PgncBTiBKVrrpUExtASR6sEMAWprrXsBzwCvelYopdoBo4DeQCpwo1IqJShWVp8yz9OLF4BLA2qVfynvt2wA/A0YqLXuCfwAxAbDSD9Q3nk2Bh4FegE3Av8IioWlEKkC0wf4FEBrvR7wngLzINBfa+1wP+ligMLAm+gXyjtPlFLDcT3xPg28aX6jvHPsDWwHXlVKfQUc0VrnBd5Ev1DeeZ4BsoB67pcz4NaVQaQKTEPghNdnh1IqGkBrbddaH1VKWZRSrwCbtda7g2Jl9SnzPJVSybhc6j8EwzA/UuY54vJWrgP+D7gZeEwpZQuwff6ivPME14MxA9gEvBZIw8ojUgXmJNDA67NVa13k+aCUqg3Md2/zcIBt8yflneevgQRgNXAf8IRSqn9gzfML5Z3jMeB7rXWO1vo08D+gW6AN9BPlnefNQAugLdAaGKKU6hFg+0olUgVmLXALgFIqFZcbjfuzBVgObNVaP6i1dgTHRL9Q5nlqrSdprXtqrfsB7wJ/11qHY6hU5jnieponK6Vi3U/7VFxP+XCkvPM8DhQA57TWhUA+0DjgFpZCRHZ29MrIp+BqXbgf14+3F4gCFgDrvXZ5Vmv9TaDtrC7lnafW+t9e2/0RyAnzVqRSz1EpNQJ42r35h1rrl4NjafXw4TwnA/1x5V++BiaFQmtZRAqMIAiBIVJDJEEQAoAIjCAIpiECIwiCaYjACIJgGiIwgiCYhgiMUCFKqfuUUi/5uG0/pdRC9/sJSqmdSqk7fdy3uVJqRinLX1JK3ec5ZmVtEoJHRPamFgLGUOAOrfX2CrcEtNY5VFw5/RzwenUNEwKD1MEIFeL2Hu4DzgNxwBta6zfdnSXH4+oQagC3AcnAQ7i6IPwV2A3cqbXOdB9rI67S9uO4Svn7aa03KaU2ASOBuVrrVKXUMFxikgdcgqvaOAHX0AtvA9+VZpOZ10GoPBIiCb5iB27CJSKPuZfZgAFa6z64SvBv8mzsvtm3AL/2iIub5e7t+gCZwA1KqSRcQnQOQCkVA/wduMG97Vn3Mf8C/KS19ng5pdkkhBAiMIKvbHKXnucAdd3LcoG5Sql3cJWwx/hwnCW4Stz7A7/DJSKDgDSvbeJwCckx93euq4RNQgghAiP4ygWxtFKqETAZ18h/Y3F1trNUdBCtdTrQDugBrATqA4Pd7z3kAo2VUnHuz1d7rfP+DonvQxwRGKGqnMTVw/cb4CtcAtPSx33XAHlaayfwJZCrtT7jWekehmAC8JlS6nNcORgPGUqpedU3XwgEkuQVBME0xIMRBME0RGAEQTANERhBEExDBEYQBNMQgREEwTREYARBMA0RGEEQTOP/AbIa4Umn0e69AAAAAElFTkSuQmCC\n", - "text/plain": [ - "
" - ] - }, - "metadata": { - "needs_background": "light" - }, - "output_type": "display_data" - } - ], - "source": [ - "plt.figure(figsize=figsize_gen)\n", - "\n", - "size = 5\n", - "mew = .5\n", - "marker_bs = '.'\n", - "marker_ns = '+'\n", - "\n", - "plt.scatter(\n", - " results.query('bs==0')['half_width'], \n", - " results.query('bs==0')['peak_to_trough'], \n", - " c=color_ns, s=size, marker=marker_ns, linewidth=mew, label='NS')\n", - "\n", - "plt.scatter(\n", - " results.query('bs==1')['half_width'], \n", - " results.query('bs==1')['peak_to_trough'], \n", - " c=color_bs, s=size, marker=marker_bs, linewidth=mew, label='BS')\n", - "\n", - "plt.scatter(\n", - " results.query('bs==1 and average_firing_rate > 10')['half_width'], \n", - " results.query('bs==1 and average_firing_rate > 10')['peak_to_trough'], \n", - " c='red', s=size, marker=marker_bs, linewidth=mew, label='BS rate > 10 Hz')\n", - "\n", - "plt.xlabel('half width')\n", - "plt.ylabel('peak to through')\n", - "\n", - "plt.legend(bbox_to_anchor=(0,1.02,1,0.2), loc=\"lower left\",\n", - " mode=\"expand\", borderaxespad=0, ncol=2)\n", - "\n", - "plt.savefig(output_path / \"figures\" / \"clusters_and_rate.svg\", bbox_inches=\"tight\")\n", - "plt.savefig(output_path / \"figures\" / \"clusters_and_rate.png\", dpi=600, bbox_inches=\"tight\")" - ] - }, - { - "cell_type": "code", - "execution_count": 31, - "metadata": {}, - "outputs": [], - "source": [ - "stim = results.query('stimulated').loc[:, ['half_width', 'peak_to_trough']].dropna()\n", - "\n", - "idxs_stim = cluster_waveform_features(stim.half_width, stim.peak_to_trough)\n", - "\n", - "results.loc[stim.index, 'bs_stim'] = idxs_stim" - ] - }, - { - "cell_type": "code", - "execution_count": 32, - "metadata": {}, - "outputs": [], - "source": [ - "control = results.query('not stimulated').loc[:, ['half_width', 'peak_to_trough']].dropna()\n", - "\n", - "idxs_control = cluster_waveform_features(control.half_width, control.peak_to_trough)\n", - "\n", - "results.loc[control.index, 'bs_ctrl'] = idxs_control" - ] - }, - { - "cell_type": "code", - "execution_count": 87, - "metadata": {}, - "outputs": [ - { - "data": { - "image/png": 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\n", - "text/plain": [ - "
" - ] - }, - "metadata": { - "needs_background": "light" - }, - "output_type": "display_data" - } - ], - "source": [ - "plt.figure(figsize=figsize_gen)\n", - "size = 5\n", - "mew = .5\n", - "marker_bs = '.'\n", - "marker_ns = '+'\n", - "plt.scatter(\n", - " results.query('bs_stim==0')['half_width'], \n", - " results.query('bs_stim==0')['peak_to_trough'], \n", - " c=color_stimulated, s=size, marker=marker_ns, \n", - " linewidth=mew, label='Stimulated NS', alpha=.5)\n", - "\n", - "plt.scatter(\n", - " results.query('bs_stim==1')['half_width'], \n", - " results.query('bs_stim==1')['peak_to_trough'], \n", - " c=color_stimulated, s=size, marker=marker_bs, \n", - " linewidth=mew, label='Stimulated BS', alpha=.5)\n", - "\n", - "\n", - "plt.scatter(\n", - " results.query('bs_ctrl==0')['half_width'], \n", - " results.query('bs_ctrl==0')['peak_to_trough'], \n", - " c=color_control, s=size, marker=marker_ns, \n", - " linewidth=mew, label='Control NS', alpha=.5)\n", - "\n", - "plt.scatter(\n", - " results.query('bs_ctrl==1')['half_width'], \n", - " results.query('bs_ctrl==1')['peak_to_trough'], \n", - " c=color_control, s=size, marker=marker_bs, \n", - " linewidth=mew, label='Control BS', alpha=.5)\n", - "\n", - "plt.xlabel('half width')\n", - "plt.ylabel('peak to through')\n", - "plt.legend(bbox_to_anchor=(0,1.02,1,0.2), loc=\"lower left\",\n", - " mode=\"expand\", borderaxespad=0, ncol=2)\n", - "\n", - "plt.savefig(output_path / \"figures\" / \"compare-clusters.svg\", bbox_inches=\"tight\")\n", - "plt.savefig(output_path / \"figures\" / \"compare-clusters.png\", dpi=600, bbox_inches=\"tight\")" - ] - }, - { - "cell_type": "code", - "execution_count": 47, - "metadata": {}, - "outputs": [], - "source": [ - "results.average_firing_rate = results.apply(lambda x: float(x.average_firing_rate), axis=1)" - ] - }, - { - "cell_type": "code", - "execution_count": 64, - "metadata": {}, - "outputs": [], - "source": [ - "results.frequency = results.apply(\n", - " lambda x: \n", - " float(x.frequency.replace('Hz', '')) if isinstance(x.frequency, str) \n", - " else float(x.frequency), axis=1)" - ] - }, - { - "cell_type": "code", - "execution_count": 74, - "metadata": {}, - "outputs": [ - { - "data": { - "image/png": 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\n", - "text/plain": [ - "
" - ] - }, - "metadata": { - "needs_background": "light" - }, - "output_type": "display_data" - } - ], - "source": [ - "bins=100\n", - "density=True\n", - "cumulative=True\n", - "histtype='step'\n", - "lw = 2\n", - "\n", - "plt.figure(figsize=figsize_gen)\n", - "plt.title('Narrow spiking')\n", - "_, bins, _ = plt.hist(\n", - " results.query('bs_ctrl==0')['average_firing_rate'], \n", - " bins=bins, density=density, cumulative=cumulative, \n", - " histtype=histtype, color=color_control, lw=lw, label='Control');\n", - "\n", - "_, bins, _ = plt.hist(\n", - " results.query('bs_stim==0')['average_firing_rate'], \n", - " bins=bins, density=density, cumulative=cumulative, \n", - " histtype=histtype, color=color_stimulated, lw=lw, label='Stimulated');\n", - "\n", - "plt.xlim(-.5, 56)\n", - "# plt.legend(bbox_to_anchor=(0,1.02,1,0.2), loc=\"lower left\",\n", - "# mode=\"expand\", borderaxespad=0, ncol=2)\n", - "\n", - "plt.savefig(output_path / \"figures\" / \"cumulative_ns.svg\", bbox_inches=\"tight\")\n", - "plt.savefig(output_path / \"figures\" / \"cumulative_ns.png\", dpi=600, bbox_inches=\"tight\")" - ] - }, - { - "cell_type": "code", - "execution_count": 75, - "metadata": {}, - "outputs": [ - { - "data": { - "image/png": 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\n", - "text/plain": [ - "
" - ] - }, - "metadata": { - "needs_background": "light" - }, - "output_type": "display_data" - } - ], - "source": [ - "bins=100\n", - "density=True\n", - "cumulative=True\n", - "histtype='step'\n", - "lw = 2\n", - "\n", - "plt.figure(figsize=figsize_gen)\n", - "plt.title('Narrow spiking')\n", - "_, bins, _ = plt.hist(\n", - " results.query('bs_ctrl==0')['average_firing_rate'], \n", - " bins=bins, density=density, cumulative=cumulative, \n", - " histtype=histtype, color=color_control, lw=lw, label='Control');\n", - "\n", - "_, bins, _ = plt.hist(\n", - " results.query('bs_stim==0 and frequency==30')['average_firing_rate'], \n", - " bins=bins, density=density, cumulative=cumulative, \n", - " histtype=histtype, color=color_stimulated, lw=lw, label='Stimulated');\n", - "\n", - "plt.xlim(-.5, 56)\n", - "# plt.legend(bbox_to_anchor=(0,1.02,1,0.2), loc=\"lower left\",\n", - "# mode=\"expand\", borderaxespad=0, ncol=2)\n", - "\n", - "plt.savefig(output_path / \"figures\" / \"cumulative_ns_30.svg\", bbox_inches=\"tight\")\n", - "plt.savefig(output_path / \"figures\" / \"cumulative_ns_30.png\", dpi=600, bbox_inches=\"tight\")" - ] - }, - { - "cell_type": "code", - "execution_count": 76, - "metadata": {}, - "outputs": [ - { - "data": { - "image/png": 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\n", - "text/plain": [ - "
" - ] - }, - "metadata": { - "needs_background": "light" - }, - "output_type": "display_data" - } - ], - "source": [ - "bins=100\n", - "density=True\n", - "cumulative=True\n", - "histtype='step'\n", - "lw = 2\n", - "\n", - "plt.figure(figsize=figsize_gen)\n", - "plt.title('Narrow spiking')\n", - "_, bins, _ = plt.hist(\n", - " results.query('bs_ctrl==0')['average_firing_rate'], \n", - " bins=bins, density=density, cumulative=cumulative, \n", - " histtype=histtype, color=color_control, lw=lw, label='Control');\n", - "\n", - "_, bins, _ = plt.hist(\n", - " results.query('bs_stim==0 and frequency==11')['average_firing_rate'], \n", - " bins=bins, density=density, cumulative=cumulative, \n", - " histtype=histtype, color=color_stimulated, lw=lw, label='Stimulated');\n", - "\n", - "plt.xlim(-.5, 56)\n", - "# plt.legend(bbox_to_anchor=(0,1.02,1,0.2), loc=\"lower left\",\n", - "# mode=\"expand\", borderaxespad=0, ncol=2)\n", - "\n", - "plt.savefig(output_path / \"figures\" / \"cumulative_ns_11.svg\", bbox_inches=\"tight\")\n", - "plt.savefig(output_path / \"figures\" / \"cumulative_ns_11.png\", dpi=600, bbox_inches=\"tight\")" - ] - }, - { - "cell_type": "code", - "execution_count": 78, - "metadata": {}, - "outputs": [ - { - "data": { - "image/png": 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I7gP8Ryp4HGY2BVji7rMiTxdyHEWEQqcZklUU7eN1ndFZJmb2aeBvwJXufhUVPhZ3PxDYiGB8YdXIS1U5joOAnczsHmAz4A/AepHXe3YcRYRCpxmSVfSEmU0Mf94NuL/AtiRmZusDdwLHufuM8OnKHYuZfdfMjg8fvkUQbI9V7TjcfXt338HdJwLzgAOAO4o4jiLK9hsJEvFBPpwhWWXHApea2UeARQSleBWcAKwNnGxmjbGFHwEXVOxYZgK/N7P7gJHAUQRtr+K/SbNC/t/SjEYRidHkJRGJUSiISIxCQURiFAoiEqNQEJEYhYKIxCgURCRGoSAiMf8DfIRJ9+bzyXgAAAAASUVORK5CYII=\n", - "text/plain": [ - "
" - ] - }, - "metadata": { - "needs_background": "light" - }, - "output_type": "display_data" - } - ], - "source": [ - "bins = 100\n", - "\n", - "plt.figure(figsize=figsize_gen)\n", - "plt.title('Broad spiking')\n", - "_, bins, _ = plt.hist(\n", - " results.query('bs_ctrl==1')['average_firing_rate'], \n", - " bins=bins, density=density, cumulative=cumulative, \n", - " histtype=histtype, color=color_control, lw=lw);\n", - "\n", - "_, bins, _ = plt.hist(\n", - " results.query('bs_stim==1')['average_firing_rate'], \n", - " bins=bins, density=density, cumulative=cumulative, \n", - " histtype=histtype, color=color_stimulated, lw=lw);\n", - "\n", - "plt.xlim(-.5, 44)\n", - "\n", - "plt.savefig(output_path / \"figures\" / \"cumulative_bs.svg\", bbox_inches=\"tight\")\n", - "plt.savefig(output_path / \"figures\" / \"cumulative_bs.png\", dpi=600, bbox_inches=\"tight\")" - ] - }, - { - "cell_type": "code", - "execution_count": 80, - "metadata": {}, - "outputs": [ - { - "data": { - "image/png": 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+NTyPiFgBPJiZ1zQ9PZLzGEUodJohWUfNfbxZZ3SOk4h4DfBfwNcy8wJqfC6Z+T5gN4rxhZc2vVSX83g/cHBEfAfYG/hX4FVNrw/tPEYRCp1mSNbRHRGxrPzzocANI2xL1yLi1cC1wMczc035dO3OJSL+NCJOLB8+SRFst9btPDLzgMw8MDOXAeuBo4FvjeI8RlG2X0GRiDfx4gzJOvsYcHZEvATYSFGK18FJwCuAkyOiMbbwYeAfanYulwNfjYjvAtsAH6Foex3/m7Qayf9bzmiUVOHkJUkVhoKkCkNBUoWhIKnCUJBUYShIqjAUJFUYCpIq/h+/ajaFnnwtFwAAAABJRU5ErkJggg==\n", - "text/plain": [ - "
" - ] - }, - "metadata": { - "needs_background": "light" - }, - "output_type": "display_data" - } - ], - "source": [ - "bins = 100\n", - "\n", - "plt.figure(figsize=figsize_gen)\n", - "plt.title('Broad spiking')\n", - "_, bins, _ = plt.hist(\n", - " results.query('bs_ctrl==1')['average_firing_rate'], \n", - " bins=bins, density=density, cumulative=cumulative, \n", - " histtype=histtype, color=color_control, lw=lw);\n", - "\n", - "_, bins, _ = plt.hist(\n", - " results.query('bs_stim==1 and frequency==11')['average_firing_rate'], \n", - " bins=bins, density=density, cumulative=cumulative, \n", - " histtype=histtype, color=color_stimulated, lw=lw);\n", - "\n", - "plt.xlim(-.5, 44)\n", - "\n", - "plt.savefig(output_path / \"figures\" / \"cumulative_bs_11.svg\", bbox_inches=\"tight\")\n", - "plt.savefig(output_path / \"figures\" / \"cumulative_bs_11.png\", dpi=600, bbox_inches=\"tight\")" - ] - }, - { - "cell_type": "code", - "execution_count": 79, - "metadata": {}, - "outputs": [ - { - "data": { - "image/png": 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7YWYTgcXuPivydCH7UUQotJohWUXRY7wBZ3SWiZltCvwD+JO7X0uF98XdjwS2JugvrBN5qSr7cTSwl5ndB2wP/BHYJPJ6x/ajiFBoNUOyip40s3Hhz/sBDxY4lsTM7FPA3cCp7j4jfLpy+2Jm3zGz08KH7xIE22NV2w93393d93D3ccA84Ajgr0XsRxFl+y0EiTiXVTMkq+wU4AozWwtYSFCKV8HpwCeAs8ys1ls4AfhVxfZlJnClmT0ADAVOJBh7Ff+b1Cvk/y3NaBSRGE1eEpEYhYKIxCgURCRGoSAiMQoFEYlRKIhIjEJBRGIUCiIS838mVz6OR2terAAAAABJRU5ErkJggg==\n", - "text/plain": [ - "
" - ] - }, - "metadata": { - "needs_background": "light" - }, - "output_type": "display_data" - } - ], - "source": [ - "bins = 100\n", - "\n", - "plt.figure(figsize=figsize_gen)\n", - "plt.title('Broad spiking')\n", - "_, bins, _ = plt.hist(\n", - " results.query('bs_ctrl==1')['average_firing_rate'], \n", - " bins=bins, density=density, cumulative=cumulative, \n", - " histtype=histtype, color=color_control, lw=lw);\n", - "\n", - "_, bins, _ = plt.hist(\n", - " results.query('bs_stim==1 and frequency==30')['average_firing_rate'], \n", - " bins=bins, density=density, cumulative=cumulative, \n", - " histtype=histtype, color=color_stimulated, lw=lw);\n", - "\n", - "plt.xlim(-.5, 44)\n", - "\n", - "plt.savefig(output_path / \"figures\" / \"cumulative_bs_30.svg\", bbox_inches=\"tight\")\n", - "plt.savefig(output_path / \"figures\" / \"cumulative_bs_30.png\", dpi=600, bbox_inches=\"tight\")" - ] - }, - { - "cell_type": "code", - "execution_count": 73, - "metadata": {}, - "outputs": [ - { - "name": "stdout", - "output_type": "stream", - "text": [ - "U-test: U value 104995.0 p value 0.047251399227811186\n", - "U-test: U value 13472.0 p value 0.6449484150738996\n" - ] - }, - { - "data": { - "image/png": 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\n", 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\n", 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" - ] - }, - "metadata": { - "needs_background": "light" - }, - "output_type": "display_data" - } - ], - "source": [ - "plt.figure(figsize=figsize_violin)\n", - "# test = 'permutation_resampling'\n", - "test = 'mann_whitney'\n", - "\n", - "plt.title('Broad spiking')\n", - "violinplot(\n", - " results.query('bs_ctrl==1')['average_firing_rate'].to_numpy(), \n", - " results.query('bs_stim==1')['average_firing_rate'].to_numpy(), \n", - " test=test)\n", - "\n", - "plt.savefig(output_path / \"figures\" / \"rates_bs.svg\", bbox_inches=\"tight\")\n", - "plt.savefig(output_path / \"figures\" / \"rates_bs.png\", dpi=600, bbox_inches=\"tight\")\n", - "\n", - "plt.figure(figsize=figsize_violin)\n", - "plt.title('Narrow spiking')\n", - "violinplot(\n", - " results.query('bs_ctrl==0')['average_firing_rate'].to_numpy(), \n", - " results.query('bs_stim==0')['average_firing_rate'].to_numpy(), \n", - " test=test)\n", - "\n", - "plt.savefig(output_path / \"figures\" / \"rates_ns.svg\", bbox_inches=\"tight\")\n", - "plt.savefig(output_path / \"figures\" / \"rates_ns.png\", dpi=600, bbox_inches=\"tight\")" - ] - }, - { - "cell_type": "code", - "execution_count": 81, - "metadata": {}, - "outputs": [ - { - "name": "stdout", - "output_type": "stream", - "text": [ - "U-test: U value 63173.0 p value 0.3220377333457417\n", - "U-test: U value 8043.0 p value 0.32905688838219194\n" - ] - }, - { - "data": { - "image/png": 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\n", 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\n", 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" - ] - }, - "metadata": { - "needs_background": "light" - }, - "output_type": "display_data" - } - ], - "source": [ - "plt.figure(figsize=figsize_violin)\n", - "# test = 'permutation_resampling'\n", - "test = 'mann_whitney'\n", - "\n", - "plt.title('Broad spiking')\n", - "violinplot(\n", - " results.query('bs_ctrl==1')['average_firing_rate'].to_numpy(), \n", - " results.query('bs_stim==1 and frequency==11')['average_firing_rate'].to_numpy(), \n", - " test=test)\n", - "\n", - "plt.savefig(output_path / \"figures\" / \"rates_bs_11.svg\", bbox_inches=\"tight\")\n", - "plt.savefig(output_path / \"figures\" / \"rates_bs_11.png\", dpi=600, bbox_inches=\"tight\")\n", - "\n", - "plt.figure(figsize=figsize_violin)\n", - "plt.title('Narrow spiking')\n", - "violinplot(\n", - " results.query('bs_ctrl==0')['average_firing_rate'].to_numpy(), \n", - " results.query('bs_stim==0 and frequency==11')['average_firing_rate'].to_numpy(), \n", - " test=test)\n", - "\n", - "plt.savefig(output_path / \"figures\" / \"rates_ns_11.svg\", bbox_inches=\"tight\")\n", - "plt.savefig(output_path / \"figures\" / \"rates_ns_11.png\", dpi=600, bbox_inches=\"tight\")" - ] - }, - { - "cell_type": "code", - "execution_count": 82, - "metadata": {}, - "outputs": [ - { - "name": "stdout", - "output_type": "stream", - "text": [ - "U-test: U value 41822.0 p value 0.016842133424593897\n", - "U-test: U value 5429.0 p value 0.7318233387867226\n" - ] - }, - { - "data": { - "image/png": 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\n", 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" - ] - }, - "metadata": { - "needs_background": "light" - }, - "output_type": "display_data" - } - ], - "source": [ - "plt.figure(figsize=figsize_violin)\n", - "# test = 'permutation_resampling'\n", - "test = 'mann_whitney'\n", - "\n", - "plt.title('Broad spiking')\n", - "violinplot(\n", - " results.query('bs_ctrl==1')['average_firing_rate'].to_numpy(), \n", - " results.query('bs_stim==1 and frequency==30')['average_firing_rate'].to_numpy(), \n", - " test=test)\n", - "\n", - "plt.savefig(output_path / \"figures\" / \"rates_bs.svg\", bbox_inches=\"tight\")\n", - "plt.savefig(output_path / \"figures\" / \"rates_bs.png\", dpi=600, bbox_inches=\"tight\")\n", - "\n", - "plt.figure(figsize=figsize_violin)\n", - "plt.title('Narrow spiking')\n", - "violinplot(\n", - " results.query('bs_ctrl==0')['average_firing_rate'].to_numpy(), \n", - " results.query('bs_stim==0 and frequency==30')['average_firing_rate'].to_numpy(), \n", - " test=test)\n", - "\n", - "plt.savefig(output_path / \"figures\" / \"rates_ns_30.svg\", bbox_inches=\"tight\")\n", - "plt.savefig(output_path / \"figures\" / \"rates_ns_30.png\", dpi=600, bbox_inches=\"tight\")" - ] - }, - { - "cell_type": "code", - "execution_count": 101, - "metadata": {}, - "outputs": [], - "source": [ - "columns = [\n", - " 'average_firing_rate',\n", - " 'half_width',\n", - " 'peak_to_trough'\n", - "]\n", - "\n", - "\n", - "def summarize(data):\n", - " return \"{:.2f} ± {:.2f} ({})\".format(data.mean(), data.sem(), sum(~np.isnan(data)))\n", - "\n", - "\n", - "bs = pd.DataFrame()\n", - "\n", - "bs['Control'] = results.query('bs_ctrl==1')[columns].agg(summarize)\n", - "bs['Stimulated'] = results.query('bs_stim==1')[columns].agg(summarize)\n", - "\n", - "ns = pd.DataFrame()\n", - "\n", - "ns['Control'] = results.query('bs_ctrl==0')[columns].agg(summarize)\n", - "ns['Stimulated'] = results.query('bs_stim==0')[columns].agg(summarize)\n", - "\n", - "\n", - "def MWU(column, df, cluster, extra):\n", - " '''\n", - " Mann Whitney U\n", - " '''\n", - " Uvalue, pvalue = scipy.stats.mannwhitneyu(\n", - " df.query('bs_ctrl=={} {}'.format(cluster, extra))[column].dropna(), \n", - " df.query('bs_stim=={} {}'.format(cluster, extra))[column].dropna(),\n", - " alternative='two-sided')\n", - "\n", - " return \"{:.2f}, {:.3f}\".format(Uvalue, pvalue)\n", - "\n", - "\n", - "def PRS(column, df, cluster, extra):\n", - " '''\n", - " Permutation ReSampling\n", - " '''\n", - " pvalue, observed_diff, diffs = permutation_resampling(\n", - " df.query('bs_ctrl=={} {}'.format(cluster, extra))[column].dropna(), \n", - " df.query('bs_stim=={} {}'.format(cluster, extra))[column].dropna())\n", - "\n", - " return \"{:.2f}, {:.3f}\".format(observed_diff, pvalue)\n", - "\n", - "\n", - "bs['MWU'] = list(map(lambda x: MWU(x, results, 1, ''), columns))\n", - "bs['PRS'] = list(map(lambda x: PRS(x, results, 1, ''), columns))\n", - "\n", - "ns['MWU'] = list(map(lambda x: MWU(x, results, 0, ''), columns))\n", - "ns['PRS'] = list(map(lambda x: PRS(x, results, 0, ''), columns))\n", - "\n", - "bs.to_latex(output_path / \"statistics\" / \"broad_spiking.tex\")\n", - "bs.to_csv(output_path / \"statistics\" / \"broad_spiking.csv\")\n", - "\n", - "ns.to_latex(output_path / \"statistics\" / \"narrow_spiking.tex\")\n", - "ns.to_csv(output_path / \"statistics\" / \"narrow_spiking.csv\")" - ] - }, - { - "cell_type": "code", - "execution_count": 99, - "metadata": {}, - "outputs": [], - "source": [ - "columns = [\n", - " 'average_firing_rate',\n", - " 'half_width',\n", - " 'peak_to_trough'\n", - "]\n", - "\n", - "\n", - "def summarize(data):\n", - " return \"{:.2f} ± {:.2f} ({})\".format(data.mean(), data.sem(), sum(~np.isnan(data)))\n", - "\n", - "\n", - "bs = pd.DataFrame()\n", - "\n", - "bs['Control'] = results.query('bs_ctrl==1')[columns].agg(summarize)\n", - "bs['Stimulated'] = results.query('bs_stim==1 and frequency==11')[columns].agg(summarize)\n", - "\n", - "ns = pd.DataFrame()\n", - "\n", - "ns['Control'] = results.query('bs_ctrl==0')[columns].agg(summarize)\n", - "ns['Stimulated'] = results.query('bs_stim==0 and frequency==11')[columns].agg(summarize)\n", - "\n", - "\n", - "def MWU(column, df, cluster, extra):\n", - " '''\n", - " Mann Whitney U\n", - " '''\n", - " Uvalue, pvalue = scipy.stats.mannwhitneyu(\n", - " df.query('bs_ctrl=={} {}'.format(cluster, extra))[column].dropna(), \n", - " df.query('bs_stim=={} {} and frequency==11'.format(cluster, extra))[column].dropna(),\n", - " alternative='two-sided')\n", - "\n", - " return \"{:.2f}, {:.3f}\".format(Uvalue, pvalue)\n", - "\n", - "\n", - "def PRS(column, df, cluster, extra):\n", - " '''\n", - " Permutation ReSampling\n", - " '''\n", - " pvalue, observed_diff, diffs = permutation_resampling(\n", - " df.query('bs_ctrl=={} {}'.format(cluster, extra))[column].dropna(), \n", - " df.query('bs_stim=={} {} and frequency==11'.format(cluster, extra))[column].dropna())\n", - "\n", - " return \"{:.2f}, {:.3f}\".format(observed_diff, pvalue)\n", - "\n", - "\n", - "bs['MWU'] = list(map(lambda x: MWU(x, results, 1, ''), columns))\n", - "bs['PRS'] = list(map(lambda x: PRS(x, results, 1, ''), columns))\n", - "\n", - "ns['MWU'] = list(map(lambda x: MWU(x, results, 0, ''), columns))\n", - "ns['PRS'] = list(map(lambda x: PRS(x, results, 0, ''), columns))\n", - "\n", - "bs.to_latex(output_path / \"statistics\" / \"broad_spiking_11.tex\")\n", - "bs.to_csv(output_path / \"statistics\" / \"broad_spiking_11.csv\")\n", - "\n", - "ns.to_latex(output_path / \"statistics\" / \"narrow_spiking_11.tex\")\n", - "ns.to_csv(output_path / \"statistics\" / \"narrow_spiking_11.csv\")" - ] - }, - { - "cell_type": "code", - "execution_count": 100, - "metadata": {}, - "outputs": [], - "source": [ - "columns = [\n", - " 'average_firing_rate',\n", - " 'half_width',\n", - " 'peak_to_trough'\n", - "]\n", - "\n", - "\n", - "def summarize(data):\n", - " return \"{:.2f} ± {:.2f} ({})\".format(data.mean(), data.sem(), sum(~np.isnan(data)))\n", - "\n", - "\n", - "bs = pd.DataFrame()\n", - "\n", - "bs['Control'] = results.query('bs_ctrl==1')[columns].agg(summarize)\n", - "bs['Stimulated'] = results.query('bs_stim==1 and frequency==30')[columns].agg(summarize)\n", - "\n", - "ns = pd.DataFrame()\n", - "\n", - "ns['Control'] = results.query('bs_ctrl==0')[columns].agg(summarize)\n", - "ns['Stimulated'] = results.query('bs_stim==0 and frequency==30')[columns].agg(summarize)\n", - "\n", - "\n", - "def MWU(column, df, cluster, extra):\n", - " '''\n", - " Mann Whitney U\n", - " '''\n", - " Uvalue, pvalue = scipy.stats.mannwhitneyu(\n", - " df.query('bs_ctrl=={} {}'.format(cluster, extra))[column].dropna(), \n", - " df.query('bs_stim=={} {} and frequency==30'.format(cluster, extra))[column].dropna(),\n", - " alternative='two-sided')\n", - "\n", - " return \"{:.2f}, {:.3f}\".format(Uvalue, pvalue)\n", - "\n", - "\n", - "def PRS(column, df, cluster, extra):\n", - " '''\n", - " Permutation ReSampling\n", - " '''\n", - " pvalue, observed_diff, diffs = permutation_resampling(\n", - " df.query('bs_ctrl=={} {}'.format(cluster, extra))[column].dropna(), \n", - " df.query('bs_stim=={} {} and frequency==30'.format(cluster, extra))[column].dropna())\n", - "\n", - " return \"{:.2f}, {:.3f}\".format(observed_diff, pvalue)\n", - "\n", - "\n", - "bs['MWU'] = list(map(lambda x: MWU(x, results, 1, ''), columns))\n", - "bs['PRS'] = list(map(lambda x: PRS(x, results, 1, ''), columns))\n", - "\n", - "ns['MWU'] = list(map(lambda x: MWU(x, results, 0, ''), columns))\n", - "ns['PRS'] = list(map(lambda x: PRS(x, results, 0, ''), columns))\n", - "\n", - "bs.to_latex(output_path / \"statistics\" / \"broad_spiking_30.tex\")\n", - "bs.to_csv(output_path / \"statistics\" / \"broad_spiking_30.csv\")\n", - "\n", - "ns.to_latex(output_path / \"statistics\" / \"narrow_spiking_30.tex\")\n", - "ns.to_csv(output_path / \"statistics\" / \"narrow_spiking_30.csv\")" - ] - }, - { - "cell_type": "code", - "execution_count": 97, - "metadata": {}, - "outputs": [ - { - "data": { - "text/html": [ - "
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ControlStimulatedMWUPRS
average_firing_rate9.53 ± 0.37 (491)10.62 ± 0.44 (462)104995.00, 0.0471.09, 0.058
half_width0.30 ± 0.00 (491)0.30 ± 0.00 (462)116089.00, 0.5300.00, 0.420
peak_to_trough0.66 ± 0.00 (491)0.67 ± 0.01 (462)116944.00, 0.4070.00, 0.522
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" - ], - "text/plain": [ - " Control Stimulated MWU \\\n", - "average_firing_rate 9.53 ± 0.37 (491) 10.62 ± 0.44 (462) 104995.00, 0.047 \n", - "half_width 0.30 ± 0.00 (491) 0.30 ± 0.00 (462) 116089.00, 0.530 \n", - "peak_to_trough 0.66 ± 0.00 (491) 0.67 ± 0.01 (462) 116944.00, 0.407 \n", - "\n", - " PRS \n", - "average_firing_rate 1.09, 0.058 \n", - "half_width 0.00, 0.420 \n", - "peak_to_trough 0.00, 0.522 " - ] - }, - "execution_count": 97, - "metadata": {}, - "output_type": "execute_result" - } - ], - "source": [ - "bs" - ] - }, - { - "cell_type": "code", - "execution_count": 98, - "metadata": {}, - "outputs": [ - { - "data": { - "text/html": [ - "
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ControlStimulatedMWUPRS
average_firing_rate21.22 ± 1.17 (147)20.20 ± 0.96 (178)13472.00, 0.6451.02, 0.501
half_width0.23 ± 0.01 (147)0.23 ± 0.00 (178)12839.00, 0.7730.01, 0.454
peak_to_trough0.29 ± 0.00 (147)0.29 ± 0.00 (178)13194.50, 0.8950.00, 0.893
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" - ], - "text/plain": [ - " Control Stimulated MWU \\\n", - "average_firing_rate 21.22 ± 1.17 (147) 20.20 ± 0.96 (178) 13472.00, 0.645 \n", - "half_width 0.23 ± 0.01 (147) 0.23 ± 0.00 (178) 12839.00, 0.773 \n", - "peak_to_trough 0.29 ± 0.00 (147) 0.29 ± 0.00 (178) 13194.50, 0.895 \n", - "\n", - " PRS \n", - "average_firing_rate 1.02, 0.501 \n", - "half_width 0.01, 0.454 \n", - "peak_to_trough 0.00, 0.893 " - ] - }, - "execution_count": 98, - "metadata": {}, - "output_type": "execute_result" - } - ], - "source": [ - "ns" - ] - }, - { - "cell_type": "markdown", - "metadata": {}, - "source": [ - "# example waveforms" - ] - }, - { - "cell_type": "code", - "execution_count": 102, - "metadata": {}, - "outputs": [], - "source": [ - "def normalize(a):\n", - " t = a - a.min()\n", - " return t / t.max()" - ] - }, - { - "cell_type": "code", - "execution_count": null, - "metadata": {}, - "outputs": [], - "source": [ - "'half_width','peak_to_trough'" - ] - }, - { - "cell_type": "code", - "execution_count": 142, - "metadata": {}, - "outputs": [ - { - "data": { - "text/plain": [ - "[]" - ] - }, - "execution_count": 142, - "metadata": {}, - "output_type": "execute_result" - }, - { - "data": { - "image/png": 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" - ] - }, - "metadata": { - "needs_background": "light" - }, - "output_type": "display_data" - } - ], - "source": [ - "plt.figure(figsize=figsize_gen)\n", - "\n", - "\n", - "lw = 3\n", - "\n", - "row = results.query('bs==1').sort_values('half_width', ascending=False).iloc[50]\n", - "template = data_loader.template(\n", - " row.action, row.channel_group, row.unit_name)\n", - "\n", - "mean_wf = template.data\n", - "peak_wf = mean_wf[np.argmin(mean_wf.min(1))]\n", - "plt.plot(normalize(peak_wf.T), color=color_bs, lw=lw)\n", - "\n", - "\n", - "row = results.query('bs==0').sort_values('half_width').iloc[10]\n", - "template = data_loader.template(\n", - " row.action, row.channel_group, row.unit_name)\n", - "\n", - "mean_wf = template.data\n", - "peak_wf = mean_wf[np.argmin(mean_wf.min(1))]\n", - "plt.plot(normalize(peak_wf.T), color=color_ns, lw=lw)\n", - "\n", - "# plt.savefig(output_path / \"figures\" / \"example_waveforms.svg\", bbox_inches=\"tight\")\n", - "# plt.savefig(output_path / \"figures\" / \"example_waveforms.png\", dpi=600, bbox_inches=\"tight\")" - ] - }, - { - "cell_type": "markdown", - "metadata": {}, - "source": [ - "# Store results in Expipe action" - ] - }, - { - "cell_type": "code", - "execution_count": 105, - "metadata": {}, - "outputs": [], - "source": [ - "action = project.require_action(\"waveform-analysis\")" - ] - }, - { - "cell_type": "code", - "execution_count": 106, - "metadata": {}, - "outputs": [], - "source": [ - "stuff = {\n", - " \"figures\": \"figures\",\n", - " \"statistics\": \"statistics\"\n", - "}\n", - "\n", - "for key, value in stuff.items():\n", - " action.data[key] = value\n", - " data_path = action.data_path(key)\n", - " data_path.parent.mkdir(exist_ok=True, parents=True)\n", - " source = output_path / value\n", - " if source.is_file():\n", - " shutil.copy(source, data_path)\n", - " else:\n", - " copy_tree(str(source), str(data_path))" - ] - }, - { - "cell_type": "code", - "execution_count": 108, - "metadata": {}, - "outputs": [], - "source": [ - "septum_mec.analysis.registration.store_notebook(action, \"10_waveform_analysis.ipynb\")" - ] - } - ], - "metadata": { - "kernelspec": { - "display_name": "Python 3", - "language": "python", - "name": "python3" - }, - "language_info": { - "codemirror_mode": { - "name": "ipython", - "version": 3 - }, - "file_extension": ".py", - "mimetype": "text/x-python", - "name": "python", - "nbconvert_exporter": "python", - "pygments_lexer": "ipython3", - "version": "3.6.8" - } - }, - "nbformat": 4, - "nbformat_minor": 2 -} +{ + "cells": [ + { + "cell_type": "code", + "execution_count": 1, + "metadata": {}, + "outputs": [], + "source": [ + "%load_ext autoreload\n", + "%autoreload 2" + ] + }, + { + "cell_type": "code", + "execution_count": 2, + "metadata": {}, + "outputs": [ + { + "name": "stderr", + "output_type": "stream", + "text": [ + "14:00:33 [I] klustakwik KlustaKwik2 version 0.2.6\n" + ] + } + ], + "source": [ + "import os\n", + "import expipe\n", + "import pathlib\n", + "import numpy as np\n", + "import spatial_maps.stats as stats\n", + "import septum_mec\n", + "import septum_mec.analysis.data_processing as dp\n", + "import septum_mec.analysis.registration\n", + "import head_direction.head as head\n", + "import spatial_maps as sp\n", + "import speed_cells.speed as spd\n", + "import re\n", + "import joblib\n", + "import multiprocessing\n", + "import shutil\n", + "import psutil\n", + "import pandas as pd\n", + "import matplotlib.pyplot as plt\n", + "from distutils.dir_util import copy_tree\n", + "from neo import SpikeTrain\n", + "import scipy\n", + "\n", + "from tqdm import tqdm_notebook as tqdm\n", + "from tqdm._tqdm_notebook import tqdm_notebook\n", + "tqdm_notebook.pandas()\n", + "\n", + "from spike_statistics.core import permutation_resampling\n", + "\n", + "from spikewaveform.core import calculate_waveform_features_from_template, cluster_waveform_features\n", + "\n", + "from septum_mec.analysis.plotting import violinplot" + ] + }, + { + "cell_type": "code", + "execution_count": 3, + "metadata": {}, + "outputs": [], + "source": [ + "%matplotlib inline\n", + "color_control = '#4393c3'\n", + "color_stimulated = '#d6604d'\n", + "\n", + "color_bs = '#5aae61'\n", + "color_ns = '#9970ab'\n", + "\n", + "figsize_violin = (1.7, 3)\n", + "figsize_gen = (4, 3)\n", + "\n", + "output_path = pathlib.Path(\"output\") / \"waveform-analysis\"\n", + "(output_path / \"statistics\").mkdir(exist_ok=True, parents=True)\n", + "(output_path / \"figures\").mkdir(exist_ok=True, parents=True)\n", + "output_path.mkdir(exist_ok=True)" + ] + }, + { + "cell_type": "code", + "execution_count": 4, + "metadata": {}, + "outputs": [], + "source": [ + "data_loader = dp.Data()\n", + "actions = data_loader.actions\n", + "project = data_loader.project" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "N cells: 1298\n" + ] + } + ], + "source": [ + "identify_neurons = actions['identify-neurons']\n", + "sessions = pd.read_csv(identify_neurons.data_path('sessions'))\n", + "units = pd.read_csv(identify_neurons.data_path('all_non_identified_units'))\n", + "# units = pd.read_csv(identify_neurons.data_path('units'))\n", + "session_units = pd.merge(sessions, units, on='action')\n", + "#########################3\n", + "# session_units = session_units.drop_duplicates('unit_id')\n", + "#################################\n", + "print('N cells:',session_units.shape[0])" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [ + { + "data": { + "text/html": [ + "
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actionbaselineentityfrequencyiiisessionstim_locationstimulatedtagchannel_groupunit_name
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" + ], + "text/plain": [ + " action baseline entity frequency i ii session \\\n", + "0 1849-060319-3 True 1849 NaN False True 3 \n", + "1 1849-060319-3 True 1849 NaN False True 3 \n", + "2 1849-060319-3 True 1849 NaN False True 3 \n", + "3 1849-060319-3 True 1849 NaN False True 3 \n", + "4 1849-060319-3 True 1849 NaN False True 3 \n", + "\n", + " stim_location stimulated tag channel_group unit_name \n", + "0 NaN False baseline ii 1 104 \n", + "1 NaN False baseline ii 1 108 \n", + "2 NaN False baseline ii 1 85 \n", + "3 NaN False baseline ii 1 94 \n", + "4 NaN False baseline ii 1 98 " + ] + }, + "execution_count": 6, + "metadata": {}, + "output_type": "execute_result" + } + ], + "source": [ + "session_units.head()" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [ + { + "data": { + "text/plain": [ + "action\n", + "1849-110319-2 13\n", + "1849-010319-4 13\n", + "1849-220319-4 13\n", + "1834-220319-3 13\n", + "1833-200619-1 13\n", + "1849-220319-3 13\n", + "1849-060319-3 13\n", + "1834-110319-1 13\n", + "1833-050619-3 13\n", + "1833-120619-3 13\n", + "1833-050619-4 13\n", + "1833-060619-1 13\n", + "1834-120319-3 12\n", + "1834-120319-2 12\n", + "1839-120619-3 12\n", + "1849-220319-5 12\n", + "1834-150319-1 12\n", + "1839-290519-1 12\n", + "1834-010319-5 11\n", + "1849-060319-4 11\n", + "1849-060319-2 11\n", + "1834-120319-4 10\n", + "1839-120619-2 10\n", + "1839-060619-5 9\n", + "1833-050619-1 9\n", + "1834-110319-5 9\n", + "1834-110319-3 9\n", + "1833-120619-4 9\n", + "1834-120319-1 8\n", + "1833-120619-1 7\n", + "1839-290519-3 6\n", + "1839-200619-1 5\n", + "1834-110319-6 5\n", + "1849-110319-3 4\n", + "1839-060619-4 4\n", + "1839-060619-3 4\n", + "1839-120619-1 3\n", + "1839-060619-1 2\n", + "Name: unit_name, dtype: int64" + ] + }, + "execution_count": 7, + "metadata": {}, + "output_type": "execute_result" + } + ], + "source": [ + "session_units.groupby('action').count().unit_name.hist()\n", + "# session_units.groupby('action').count().sort_values('unit_name', ascending=False)" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "# Process all data" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "def features(row):\n", + " action_id = row['action']\n", + " channel_id = row['channel_group']\n", + " unit = row['unit_name']\n", + " template = data_loader.template(action_id, channel_id, unit)\n", + " spike_times = data_loader.spike_train(action_id, channel_id, unit)\n", + " half_widths, peak_to_troughs = calculate_waveform_features_from_template(\n", + " template.data, template.sampling_rate)\n", + " peak_amps = template.data.min(axis=1)\n", + " half_widths = half_widths * 1000 # to ms\n", + " peak_to_troughs = peak_to_troughs * 1000 # to ms\n", + " idxs = np.argsort(peak_amps)\n", + " peak_to_trough = np.nan\n", + " for p2t in peak_to_troughs[idxs]:\n", + " if np.isfinite(p2t) and p2t > .1:\n", + " peak_to_trough = p2t\n", + " break\n", + " half_width = np.nan\n", + " for hw in half_widths[idxs]:\n", + " if np.isfinite(hw):\n", + " half_width = hw\n", + " break\n", + " \n", + " return pd.Series({\n", + " 'half_width': half_width,\n", + " 'peak_to_trough': peak_to_trough,\n", + " 'average_firing_rate': float(len(spike_times) / spike_times.t_stop),\n", + " 'template': template.data[idxs[0]]\n", + " })" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [ + { + "data": { + "application/vnd.jupyter.widget-view+json": { + "model_id": "fb8ac000ef5b473a833b96cbfd854d1f", + "version_major": 2, + "version_minor": 0 + }, + "text/plain": [ + "HBox(children=(IntProgress(value=0, max=1298), HTML(value='')))" + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "results = session_units.merge(\n", + " session_units.progress_apply(features, axis=1), \n", + " left_index=True, right_index=True)" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "%debug" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "df = results.loc[:, ['half_width', 'peak_to_trough']].dropna()\n", + "\n", + "idxs_df = cluster_waveform_features(df.half_width, df.peak_to_trough)\n", + "\n", + "results.loc[df.index, 'bs'] = idxs_df" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "plt.figure(figsize=figsize_gen)\n", + "size = 5\n", + "mew = .5\n", + "marker_bs = '.'\n", + "marker_ns = '+'\n", + "\n", + "plt.scatter(\n", + " results.query('bs==0')['half_width'], \n", + " results.query('bs==0')['peak_to_trough'], \n", + " c=color_ns, s=size, marker=marker_ns, linewidth=mew, label='NS')\n", + "\n", + "plt.scatter(\n", + " results.query('bs==1')['half_width'], \n", + " results.query('bs==1')['peak_to_trough'], \n", + " c=color_bs, s=size, marker=marker_bs, linewidth=mew, label='BS')\n", + "\n", + "plt.xlabel('half width')\n", + "plt.ylabel('peak to through')\n", + "\n", + "plt.legend(bbox_to_anchor=(0,1.02,1,0.2), loc=\"lower left\",\n", + " mode=\"expand\", borderaxespad=0, ncol=2)\n", + "\n", + "plt.savefig(output_path / \"figures\" / \"clusters.svg\", bbox_inches=\"tight\")\n", + "plt.savefig(output_path / \"figures\" / \"clusters.png\", dpi=600, bbox_inches=\"tight\")" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "plt.figure(figsize=figsize_gen)\n", + "\n", + "size = 5\n", + "mew = .5\n", + "marker_bs = '.'\n", + "marker_ns = '+'\n", + "\n", + "plt.scatter(\n", + " results.query('bs==0')['half_width'], \n", + " results.query('bs==0')['peak_to_trough'], \n", + " c=color_ns, s=size, marker=marker_ns, linewidth=mew, label='NS')\n", + "\n", + "plt.scatter(\n", + " results.query('bs==1')['half_width'], \n", + " results.query('bs==1')['peak_to_trough'], \n", + " c=color_bs, s=size, marker=marker_bs, linewidth=mew, label='BS')\n", + "\n", + "plt.scatter(\n", + " results.query('bs==1 and average_firing_rate > 10')['half_width'], \n", + " results.query('bs==1 and average_firing_rate > 10')['peak_to_trough'], \n", + " c='red', s=size, marker=marker_bs, linewidth=mew, label='BS rate > 10 Hz')\n", + "\n", + "plt.xlabel('half width')\n", + "plt.ylabel('peak to through')\n", + "\n", + "plt.legend(bbox_to_anchor=(0,1.02,1,0.2), loc=\"lower left\",\n", + " mode=\"expand\", borderaxespad=0, ncol=2)\n", + "\n", + "plt.savefig(output_path / \"figures\" / \"clusters_and_rate.svg\", bbox_inches=\"tight\")\n", + "plt.savefig(output_path / \"figures\" / \"clusters_and_rate.png\", dpi=600, bbox_inches=\"tight\")" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "stim = results.query('stimulated').loc[:, ['half_width', 'peak_to_trough']].dropna()\n", + "\n", + "idxs_stim = cluster_waveform_features(stim.half_width, stim.peak_to_trough)\n", + "\n", + "results.loc[stim.index, 'bs_stim'] = idxs_stim" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "control = results.query('not stimulated').loc[:, ['half_width', 'peak_to_trough']].dropna()\n", + "\n", + "idxs_control = cluster_waveform_features(control.half_width, control.peak_to_trough)\n", + "\n", + "results.loc[control.index, 'bs_ctrl'] = idxs_control" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "plt.figure(figsize=figsize_gen)\n", + "size = 5\n", + "mew = .5\n", + "marker_bs = '.'\n", + "marker_ns = '+'\n", + "plt.scatter(\n", + " results.query('bs_stim==0')['half_width'], \n", + " results.query('bs_stim==0')['peak_to_trough'], \n", + " c=color_stimulated, s=size, marker=marker_ns, \n", + " linewidth=mew, label='Stimulated NS', alpha=.5)\n", + "\n", + "plt.scatter(\n", + " results.query('bs_stim==1')['half_width'], \n", + " results.query('bs_stim==1')['peak_to_trough'], \n", + " c=color_stimulated, s=size, marker=marker_bs, \n", + " linewidth=mew, label='Stimulated BS', alpha=.5)\n", + "\n", + "\n", + "plt.scatter(\n", + " results.query('bs_ctrl==0')['half_width'], \n", + " results.query('bs_ctrl==0')['peak_to_trough'], \n", + " c=color_control, s=size, marker=marker_ns, \n", + " linewidth=mew, label='Control NS', alpha=.5)\n", + "\n", + "plt.scatter(\n", + " results.query('bs_ctrl==1')['half_width'], \n", + " results.query('bs_ctrl==1')['peak_to_trough'], \n", + " c=color_control, s=size, marker=marker_bs, \n", + " linewidth=mew, label='Control BS', alpha=.5)\n", + "\n", + "plt.xlabel('half width')\n", + "plt.ylabel('peak to through')\n", + "plt.legend(bbox_to_anchor=(0,1.02,1,0.2), loc=\"lower left\",\n", + " mode=\"expand\", borderaxespad=0, ncol=2)\n", + "\n", + "plt.savefig(output_path / \"figures\" / \"compare-clusters.svg\", bbox_inches=\"tight\")\n", + "plt.savefig(output_path / \"figures\" / \"compare-clusters.png\", dpi=600, bbox_inches=\"tight\")" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "results.average_firing_rate = results.apply(lambda x: float(x.average_firing_rate), axis=1)" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "results.frequency = results.apply(\n", + " lambda x: \n", + " float(x.frequency.replace('Hz', '')) if isinstance(x.frequency, str) \n", + " else float(x.frequency), axis=1)" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "bins=100\n", + "density=True\n", + "cumulative=True\n", + "histtype='step'\n", + "lw = 2\n", + "\n", + "plt.figure(figsize=figsize_gen)\n", + "plt.title('Narrow spiking')\n", + "_, bins, _ = plt.hist(\n", + " results.query('bs_ctrl==0')['average_firing_rate'], \n", + " bins=bins, density=density, cumulative=cumulative, \n", + " histtype=histtype, color=color_control, lw=lw, label='Control');\n", + "\n", + "_, bins, _ = plt.hist(\n", + " results.query('bs_stim==0')['average_firing_rate'], \n", + " bins=bins, density=density, cumulative=cumulative, \n", + " histtype=histtype, color=color_stimulated, lw=lw, label='Stimulated');\n", + "\n", + "plt.xlim(-.5, 56)\n", + "# plt.legend(bbox_to_anchor=(0,1.02,1,0.2), loc=\"lower left\",\n", + "# mode=\"expand\", borderaxespad=0, ncol=2)\n", + "\n", + "plt.savefig(output_path / \"figures\" / \"cumulative_ns.svg\", bbox_inches=\"tight\")\n", + "plt.savefig(output_path / \"figures\" / \"cumulative_ns.png\", dpi=600, bbox_inches=\"tight\")" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "bins=100\n", + "density=True\n", + "cumulative=True\n", + "histtype='step'\n", + "lw = 2\n", + "\n", + "plt.figure(figsize=figsize_gen)\n", + "plt.title('Narrow spiking')\n", + "_, bins, _ = plt.hist(\n", + " results.query('bs_ctrl==0')['average_firing_rate'], \n", + " bins=bins, density=density, cumulative=cumulative, \n", + " histtype=histtype, color=color_control, lw=lw, label='Control');\n", + "\n", + "_, bins, _ = plt.hist(\n", + " results.query('bs_stim==0 and frequency==30')['average_firing_rate'], \n", + " bins=bins, density=density, cumulative=cumulative, \n", + " histtype=histtype, color=color_stimulated, lw=lw, label='Stimulated');\n", + "\n", + "plt.xlim(-.5, 56)\n", + "# plt.legend(bbox_to_anchor=(0,1.02,1,0.2), loc=\"lower left\",\n", + "# mode=\"expand\", borderaxespad=0, ncol=2)\n", + "\n", + "plt.savefig(output_path / \"figures\" / \"cumulative_ns_30.svg\", bbox_inches=\"tight\")\n", + "plt.savefig(output_path / \"figures\" / \"cumulative_ns_30.png\", dpi=600, bbox_inches=\"tight\")" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "bins=100\n", + "density=True\n", + "cumulative=True\n", + "histtype='step'\n", + "lw = 2\n", + "\n", + "plt.figure(figsize=figsize_gen)\n", + "plt.title('Narrow spiking')\n", + "_, bins, _ = plt.hist(\n", + " results.query('bs_ctrl==0')['average_firing_rate'], \n", + " bins=bins, density=density, cumulative=cumulative, \n", + " histtype=histtype, color=color_control, lw=lw, label='Control');\n", + "\n", + "_, bins, _ = plt.hist(\n", + " results.query('bs_stim==0 and frequency==11')['average_firing_rate'], \n", + " bins=bins, density=density, cumulative=cumulative, \n", + " histtype=histtype, color=color_stimulated, lw=lw, label='Stimulated');\n", + "\n", + "plt.xlim(-.5, 56)\n", + "# plt.legend(bbox_to_anchor=(0,1.02,1,0.2), loc=\"lower left\",\n", + "# mode=\"expand\", borderaxespad=0, ncol=2)\n", + "\n", + "plt.savefig(output_path / \"figures\" / \"cumulative_ns_11.svg\", bbox_inches=\"tight\")\n", + "plt.savefig(output_path / \"figures\" / \"cumulative_ns_11.png\", dpi=600, bbox_inches=\"tight\")" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "bins = 100\n", + "\n", + "plt.figure(figsize=figsize_gen)\n", + "plt.title('Broad spiking')\n", + "_, bins, _ = plt.hist(\n", + " results.query('bs_ctrl==1')['average_firing_rate'], \n", + " bins=bins, density=density, cumulative=cumulative, \n", + " histtype=histtype, color=color_control, lw=lw);\n", + "\n", + "_, bins, _ = plt.hist(\n", + " results.query('bs_stim==1')['average_firing_rate'], \n", + " bins=bins, density=density, cumulative=cumulative, \n", + " histtype=histtype, color=color_stimulated, lw=lw);\n", + "\n", + "plt.xlim(-.5, 44)\n", + "\n", + "plt.savefig(output_path / \"figures\" / \"cumulative_bs.svg\", bbox_inches=\"tight\")\n", + "plt.savefig(output_path / \"figures\" / \"cumulative_bs.png\", dpi=600, bbox_inches=\"tight\")" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "bins = 100\n", + "\n", + "plt.figure(figsize=figsize_gen)\n", + "plt.title('Broad spiking')\n", + "_, bins, _ = plt.hist(\n", + " results.query('bs_ctrl==1')['average_firing_rate'], \n", + " bins=bins, density=density, cumulative=cumulative, \n", + " histtype=histtype, color=color_control, lw=lw);\n", + "\n", + "_, bins, _ = plt.hist(\n", + " results.query('bs_stim==1 and frequency==11')['average_firing_rate'], \n", + " bins=bins, density=density, cumulative=cumulative, \n", + " histtype=histtype, color=color_stimulated, lw=lw);\n", + "\n", + "plt.xlim(-.5, 44)\n", + "\n", + "plt.savefig(output_path / \"figures\" / \"cumulative_bs_11.svg\", bbox_inches=\"tight\")\n", + "plt.savefig(output_path / \"figures\" / \"cumulative_bs_11.png\", dpi=600, bbox_inches=\"tight\")" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "bins = 100\n", + "\n", + "plt.figure(figsize=figsize_gen)\n", + "plt.title('Broad spiking')\n", + "_, bins, _ = plt.hist(\n", + " results.query('bs_ctrl==1')['average_firing_rate'], \n", + " bins=bins, density=density, cumulative=cumulative, \n", + " histtype=histtype, color=color_control, lw=lw);\n", + "\n", + "_, bins, _ = plt.hist(\n", + " results.query('bs_stim==1 and frequency==30')['average_firing_rate'], \n", + " bins=bins, density=density, cumulative=cumulative, \n", + " histtype=histtype, color=color_stimulated, lw=lw);\n", + "\n", + "plt.xlim(-.5, 44)\n", + "\n", + "plt.savefig(output_path / \"figures\" / \"cumulative_bs_30.svg\", bbox_inches=\"tight\")\n", + "plt.savefig(output_path / \"figures\" / \"cumulative_bs_30.png\", dpi=600, bbox_inches=\"tight\")" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "plt.figure(figsize=figsize_violin)\n", + "# test = 'permutation_resampling'\n", + "test = 'mann_whitney'\n", + "\n", + "plt.title('Broad spiking')\n", + "violinplot(\n", + " results.query('bs_ctrl==1')['average_firing_rate'].to_numpy(), \n", + " results.query('bs_stim==1')['average_firing_rate'].to_numpy(), \n", + " test=test)\n", + "\n", + "plt.savefig(output_path / \"figures\" / \"rates_bs.svg\", bbox_inches=\"tight\")\n", + "plt.savefig(output_path / \"figures\" / \"rates_bs.png\", dpi=600, bbox_inches=\"tight\")\n", + "\n", + "plt.figure(figsize=figsize_violin)\n", + "plt.title('Narrow spiking')\n", + "violinplot(\n", + " results.query('bs_ctrl==0')['average_firing_rate'].to_numpy(), \n", + " results.query('bs_stim==0')['average_firing_rate'].to_numpy(), \n", + " test=test)\n", + "\n", + "plt.savefig(output_path / \"figures\" / \"rates_ns.svg\", bbox_inches=\"tight\")\n", + "plt.savefig(output_path / \"figures\" / \"rates_ns.png\", dpi=600, bbox_inches=\"tight\")" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "plt.figure(figsize=figsize_violin)\n", + "# test = 'permutation_resampling'\n", + "test = 'mann_whitney'\n", + "\n", + "plt.title('Broad spiking')\n", + "violinplot(\n", + " results.query('bs_ctrl==1')['average_firing_rate'].to_numpy(), \n", + " results.query('bs_stim==1 and frequency==11')['average_firing_rate'].to_numpy(), \n", + " test=test)\n", + "\n", + "plt.savefig(output_path / \"figures\" / \"rates_bs_11.svg\", bbox_inches=\"tight\")\n", + "plt.savefig(output_path / \"figures\" / \"rates_bs_11.png\", dpi=600, bbox_inches=\"tight\")\n", + "\n", + "plt.figure(figsize=figsize_violin)\n", + "plt.title('Narrow spiking')\n", + "violinplot(\n", + " results.query('bs_ctrl==0')['average_firing_rate'].to_numpy(), \n", + " results.query('bs_stim==0 and frequency==11')['average_firing_rate'].to_numpy(), \n", + " test=test)\n", + "\n", + "plt.savefig(output_path / \"figures\" / \"rates_ns_11.svg\", bbox_inches=\"tight\")\n", + "plt.savefig(output_path / \"figures\" / \"rates_ns_11.png\", dpi=600, bbox_inches=\"tight\")" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "plt.figure(figsize=figsize_violin)\n", + "# test = 'permutation_resampling'\n", + "test = 'mann_whitney'\n", + "\n", + "plt.title('Broad spiking')\n", + "violinplot(\n", + " results.query('bs_ctrl==1')['average_firing_rate'].to_numpy(), \n", + " results.query('bs_stim==1 and frequency==30')['average_firing_rate'].to_numpy(), \n", + " test=test)\n", + "\n", + "plt.savefig(output_path / \"figures\" / \"rates_bs.svg\", bbox_inches=\"tight\")\n", + "plt.savefig(output_path / \"figures\" / \"rates_bs.png\", dpi=600, bbox_inches=\"tight\")\n", + "\n", + "plt.figure(figsize=figsize_violin)\n", + "plt.title('Narrow spiking')\n", + "violinplot(\n", + " results.query('bs_ctrl==0')['average_firing_rate'].to_numpy(), \n", + " results.query('bs_stim==0 and frequency==30')['average_firing_rate'].to_numpy(), \n", + " test=test)\n", + "\n", + "plt.savefig(output_path / \"figures\" / \"rates_ns_30.svg\", bbox_inches=\"tight\")\n", + "plt.savefig(output_path / \"figures\" / \"rates_ns_30.png\", dpi=600, bbox_inches=\"tight\")" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "columns = [\n", + " 'average_firing_rate',\n", + " 'half_width',\n", + " 'peak_to_trough'\n", + "]\n", + "\n", + "\n", + "def summarize(data):\n", + " return \"{:.2f} ± {:.2f} ({})\".format(data.mean(), data.sem(), sum(~np.isnan(data)))\n", + "\n", + "\n", + "bs = pd.DataFrame()\n", + "\n", + "bs['Control'] = results.query('bs_ctrl==1')[columns].agg(summarize)\n", + "bs['Stimulated'] = results.query('bs_stim==1')[columns].agg(summarize)\n", + "\n", + "ns = pd.DataFrame()\n", + "\n", + "ns['Control'] = results.query('bs_ctrl==0')[columns].agg(summarize)\n", + "ns['Stimulated'] = results.query('bs_stim==0')[columns].agg(summarize)\n", + "\n", + "\n", + "def MWU(column, df, cluster, extra):\n", + " '''\n", + " Mann Whitney U\n", + " '''\n", + " Uvalue, pvalue = scipy.stats.mannwhitneyu(\n", + " df.query('bs_ctrl=={} {}'.format(cluster, extra))[column].dropna(), \n", + " df.query('bs_stim=={} {}'.format(cluster, extra))[column].dropna(),\n", + " alternative='two-sided')\n", + "\n", + " return \"{:.2f}, {:.3f}\".format(Uvalue, pvalue)\n", + "\n", + "\n", + "def PRS(column, df, cluster, extra):\n", + " '''\n", + " Permutation ReSampling\n", + " '''\n", + " pvalue, observed_diff, diffs = permutation_resampling(\n", + " df.query('bs_ctrl=={} {}'.format(cluster, extra))[column].dropna(), \n", + " df.query('bs_stim=={} {}'.format(cluster, extra))[column].dropna())\n", + "\n", + " return \"{:.2f}, {:.3f}\".format(observed_diff, pvalue)\n", + "\n", + "\n", + "bs['MWU'] = list(map(lambda x: MWU(x, results, 1, ''), columns))\n", + "bs['PRS'] = list(map(lambda x: PRS(x, results, 1, ''), columns))\n", + "\n", + "ns['MWU'] = list(map(lambda x: MWU(x, results, 0, ''), columns))\n", + "ns['PRS'] = list(map(lambda x: PRS(x, results, 0, ''), columns))\n", + "\n", + "bs.to_latex(output_path / \"statistics\" / \"broad_spiking.tex\")\n", + "bs.to_csv(output_path / \"statistics\" / \"broad_spiking.csv\")\n", + "\n", + "ns.to_latex(output_path / \"statistics\" / \"narrow_spiking.tex\")\n", + "ns.to_csv(output_path / \"statistics\" / \"narrow_spiking.csv\")" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "columns = [\n", + " 'average_firing_rate',\n", + " 'half_width',\n", + " 'peak_to_trough'\n", + "]\n", + "\n", + "\n", + "def summarize(data):\n", + " return \"{:.2f} ± {:.2f} ({})\".format(data.mean(), data.sem(), sum(~np.isnan(data)))\n", + "\n", + "\n", + "bs = pd.DataFrame()\n", + "\n", + "bs['Control'] = results.query('bs_ctrl==1')[columns].agg(summarize)\n", + "bs['Stimulated'] = results.query('bs_stim==1 and frequency==11')[columns].agg(summarize)\n", + "\n", + "ns = pd.DataFrame()\n", + "\n", + "ns['Control'] = results.query('bs_ctrl==0')[columns].agg(summarize)\n", + "ns['Stimulated'] = results.query('bs_stim==0 and frequency==11')[columns].agg(summarize)\n", + "\n", + "\n", + "def MWU(column, df, cluster, extra):\n", + " '''\n", + " Mann Whitney U\n", + " '''\n", + " Uvalue, pvalue = scipy.stats.mannwhitneyu(\n", + " df.query('bs_ctrl=={} {}'.format(cluster, extra))[column].dropna(), \n", + " df.query('bs_stim=={} {} and frequency==11'.format(cluster, extra))[column].dropna(),\n", + " alternative='two-sided')\n", + "\n", + " return \"{:.2f}, {:.3f}\".format(Uvalue, pvalue)\n", + "\n", + "\n", + "def PRS(column, df, cluster, extra):\n", + " '''\n", + " Permutation ReSampling\n", + " '''\n", + " pvalue, observed_diff, diffs = permutation_resampling(\n", + " df.query('bs_ctrl=={} {}'.format(cluster, extra))[column].dropna(), \n", + " df.query('bs_stim=={} {} and frequency==11'.format(cluster, extra))[column].dropna())\n", + "\n", + " return \"{:.2f}, {:.3f}\".format(observed_diff, pvalue)\n", + "\n", + "\n", + "bs['MWU'] = list(map(lambda x: MWU(x, results, 1, ''), columns))\n", + "bs['PRS'] = list(map(lambda x: PRS(x, results, 1, ''), columns))\n", + "\n", + "ns['MWU'] = list(map(lambda x: MWU(x, results, 0, ''), columns))\n", + "ns['PRS'] = list(map(lambda x: PRS(x, results, 0, ''), columns))\n", + "\n", + "bs.to_latex(output_path / \"statistics\" / \"broad_spiking_11.tex\")\n", + "bs.to_csv(output_path / \"statistics\" / \"broad_spiking_11.csv\")\n", + "\n", + "ns.to_latex(output_path / \"statistics\" / \"narrow_spiking_11.tex\")\n", + "ns.to_csv(output_path / \"statistics\" / \"narrow_spiking_11.csv\")" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "columns = [\n", + " 'average_firing_rate',\n", + " 'half_width',\n", + " 'peak_to_trough'\n", + "]\n", + "\n", + "\n", + "def summarize(data):\n", + " return \"{:.2f} ± {:.2f} ({})\".format(data.mean(), data.sem(), sum(~np.isnan(data)))\n", + "\n", + "\n", + "bs = pd.DataFrame()\n", + "\n", + "bs['Control'] = results.query('bs_ctrl==1')[columns].agg(summarize)\n", + "bs['Stimulated'] = results.query('bs_stim==1 and frequency==30')[columns].agg(summarize)\n", + "\n", + "ns = pd.DataFrame()\n", + "\n", + "ns['Control'] = results.query('bs_ctrl==0')[columns].agg(summarize)\n", + "ns['Stimulated'] = results.query('bs_stim==0 and frequency==30')[columns].agg(summarize)\n", + "\n", + "\n", + "def MWU(column, df, cluster, extra):\n", + " '''\n", + " Mann Whitney U\n", + " '''\n", + " Uvalue, pvalue = scipy.stats.mannwhitneyu(\n", + " df.query('bs_ctrl=={} {}'.format(cluster, extra))[column].dropna(), \n", + " df.query('bs_stim=={} {} and frequency==30'.format(cluster, extra))[column].dropna(),\n", + " alternative='two-sided')\n", + "\n", + " return \"{:.2f}, {:.3f}\".format(Uvalue, pvalue)\n", + "\n", + "\n", + "def PRS(column, df, cluster, extra):\n", + " '''\n", + " Permutation ReSampling\n", + " '''\n", + " pvalue, observed_diff, diffs = permutation_resampling(\n", + " df.query('bs_ctrl=={} {}'.format(cluster, extra))[column].dropna(), \n", + " df.query('bs_stim=={} {} and frequency==30'.format(cluster, extra))[column].dropna())\n", + "\n", + " return \"{:.2f}, {:.3f}\".format(observed_diff, pvalue)\n", + "\n", + "\n", + "bs['MWU'] = list(map(lambda x: MWU(x, results, 1, ''), columns))\n", + "bs['PRS'] = list(map(lambda x: PRS(x, results, 1, ''), columns))\n", + "\n", + "ns['MWU'] = list(map(lambda x: MWU(x, results, 0, ''), columns))\n", + "ns['PRS'] = list(map(lambda x: PRS(x, results, 0, ''), columns))\n", + "\n", + "bs.to_latex(output_path / \"statistics\" / \"broad_spiking_30.tex\")\n", + "bs.to_csv(output_path / \"statistics\" / \"broad_spiking_30.csv\")\n", + "\n", + "ns.to_latex(output_path / \"statistics\" / \"narrow_spiking_30.tex\")\n", + "ns.to_csv(output_path / \"statistics\" / \"narrow_spiking_30.csv\")" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "bs" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "ns" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "# example waveforms" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "def normalize(a):\n", + " t = a - a.min()\n", + " return t / t.max()" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "'half_width','peak_to_trough'" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "plt.figure(figsize=figsize_gen)\n", + "\n", + "\n", + "lw = 3\n", + "\n", + "row = results.query('bs==1').sort_values('half_width', ascending=False).iloc[50]\n", + "template = data_loader.template(\n", + " row.action, row.channel_group, row.unit_name)\n", + "\n", + "mean_wf = template.data\n", + "peak_wf = mean_wf[np.argmin(mean_wf.min(1))]\n", + "plt.plot(normalize(peak_wf.T), color=color_bs, lw=lw)\n", + "\n", + "\n", + "row = results.query('bs==0').sort_values('half_width').iloc[10]\n", + "template = data_loader.template(\n", + " row.action, row.channel_group, row.unit_name)\n", + "\n", + "mean_wf = template.data\n", + "peak_wf = mean_wf[np.argmin(mean_wf.min(1))]\n", + "plt.plot(normalize(peak_wf.T), color=color_ns, lw=lw)\n", + "\n", + "plt.savefig(output_path / \"figures\" / \"example_waveforms.svg\", bbox_inches=\"tight\")\n", + "plt.savefig(output_path / \"figures\" / \"example_waveforms.png\", dpi=600, bbox_inches=\"tight\")" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "# Store results in Expipe action" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "action = project.require_action(\"waveform-analysis\")" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "action.data['results'] = 'results.csv'\n", + "results.to_csv(action.data_path('results'), index=False)" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "stuff = {\n", + " \"figures\": \"figures\",\n", + " \"statistics\": \"statistics\"\n", + "}\n", + "\n", + "for key, value in stuff.items():\n", + " action.data[key] = value\n", + " data_path = action.data_path(key)\n", + " data_path.parent.mkdir(exist_ok=True, parents=True)\n", + " source = output_path / value\n", + " if source.is_file():\n", + " shutil.copy(source, data_path)\n", + " else:\n", + " copy_tree(str(source), str(data_path))" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "septum_mec.analysis.registration.store_notebook(action, \"10_waveform_analysis.ipynb\")" + ] + } + ], + "metadata": { + "kernelspec": { + 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ii,7,28,0.34104467257862103,0.8486182221097581,9.497308110449632,"[ 3.27428878 3.14009662 3.10048309 3.04863124 2.73580247 + 1.90821256 0.34847021 -2.11111111 -5.60268384 -10.12173913 + -15.4201825 -20.88921095 -25.74943639 -29.29736983 -31.14052603 + -31.19355878 -29.64433709 -26.92785829 -23.52463768 -19.90488459 + -16.42834138 -13.27407407 -10.55373054 -8.2359635 -6.21696189 + -4.41449275 -2.69554482 -1.02812668 0.62855609 2.29457864 + 3.88094471 5.37165862 6.71680086 7.85861514 8.85732689 + 9.66945786 10.29608159 10.74761138 11.05711218 11.23692968 + 11.29243156 11.24938272 11.15147611 10.98325282 10.76768653 + 10.51519055 10.22114868 9.89092861 9.50112721 9.04562534 + 8.47525497 7.83005904 7.13140097 6.35083199 5.53032743 + 4.69533011 3.89457864 3.17058508 2.51755233 1.93268921 + 1.44240472 1.01835749 0.62683843 0.23939882 -0.12742888 + -0.54406871 -0.99452496 -1.45485776 -1.9410628 -2.41889426 + -2.87965647 -3.29039184 -3.65464305 -3.94986581 -4.19924852]",1.0,,1.0 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ii,6,83,0.23011305940476143,0.3007167478929637,33.80010080905022,"[ 2.58183234e+00 1.91305618e+00 1.59387272e+00 1.77649044e+00 + 2.41654496e+00 3.18806997e+00 3.54399117e+00 2.80803557e+00 + 3.50339143e-01 -4.17375783e+00 -1.08196216e+01 -1.91284198e+01 + -2.77915815e+01 -3.50654269e+01 -3.93648136e+01 -3.97278097e+01 + -3.59956187e+01 -2.88759290e+01 -1.97571155e+01 -1.04907993e+01 + -2.63044819e+00 3.21860903e+00 6.92980236e+00 8.63479700e+00 + 8.79164638e+00 8.06270081e+00 7.08188102e+00 6.26907474e+00 + 5.77321261e+00 5.58942654e+00 5.57177165e+00 5.58358485e+00 + 5.52383734e+00 5.36309350e+00 5.12858209e+00 4.87813585e+00 + 4.69963976e+00 4.60500438e+00 4.60490702e+00 4.65209490e+00 + 4.72518093e+00 4.76311946e+00 4.74939149e+00 4.65920228e+00 + 4.51380911e+00 4.32437608e+00 4.11686626e+00 3.91529549e+00 + 3.71466589e+00 3.52896505e+00 3.34216078e+00 3.14503619e+00 + 2.90721449e+00 2.65790413e+00 2.37711356e+00 2.08525622e+00 + 1.78950443e+00 1.48989063e+00 1.20007789e+00 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1.04706597e+01 9.82503710e+00 9.14245245e+00 8.49318764e+00 + 7.85592877e+00 7.22932686e+00 6.57709429e+00 5.87805207e+00 + 5.19991906e+00 4.49588561e+00 3.78551194e+00 3.03318495e+00 + 2.30365574e+00 1.63118845e+00 1.04330231e+00 4.93727236e-01 + 2.22581951e-02 -3.66113584e-01 -7.00526103e-01 -1.04829354e+00 + -1.34695805e+00 -1.60501821e+00 -1.84635100e+00 -2.11102118e+00 + -2.41049508e+00 -2.67516525e+00 -2.91096722e+00 -3.13611224e+00 + -3.34466478e+00 -3.57048428e+00 -3.71415082e+00]",1.0,,1.0 +1834-060319-3,True,1834,,False,True,3,,False,baseline ii,6,30,0.28312894306043357,0.719845340731475,14.94576360883967,"[ 6.88294375 6.89061359 7.2147553 7.79401023 8.26323959 + 8.11879109 6.73256026 3.57076333 -1.57213294 -8.60975164 + -17.45717677 -27.57131118 -37.48347334 -45.55076698 -50.54282323 + -51.85052958 -49.456355 -44.02784879 -36.6116691 -28.4514244 + -20.82231556 -14.54665814 -9.81418919 -6.3811176 -3.8992878 + -1.98219503 -0.32907232 1.37426954 3.20443755 5.20242878 + 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9.92664748 9.7561427 + 9.54230492 9.31551352 9.03248689 8.68381824 8.29438676 + 7.85123882 7.37066927 6.88547342 6.39452041 5.89359515 + 5.37961345 4.85488845 4.34106096 3.8291354 3.32862136 + 2.83664028 2.34491621 1.89215585 1.48277989 1.10491416 + 0.74565642 0.39380076 0.07931531 -0.20694973 -0.47784517 + -0.73563277 -1.00760769 -1.25490902 -1.49974298 -1.74293204 + -1.99121003 -2.21337514 -2.40469826 -2.58897913 -2.73321682]",1.0,,1.0 +1834-220319-3,True,1834,,False,True,3,,False,baseline ii,7,23,0.3209314068945433,0.7901523736678938,20.05632576551337,"[ 6.72047201e+00 6.42705818e+00 6.20653128e+00 6.08150384e+00 + 5.91108672e+00 5.50472009e+00 4.56844127e+00 2.81042810e+00 + -1.55323820e-02 -4.02963776e+00 -9.23962678e+00 -1.54452250e+01 + -2.22287596e+01 -2.88336992e+01 -3.42469265e+01 -3.76443469e+01 + -3.86244237e+01 -3.72547750e+01 -3.39915477e+01 -2.95586718e+01 + -2.47351811e+01 -2.01202525e+01 -1.60391877e+01 -1.25796378e+01 + -9.71509330e+00 -7.28863886e+00 -5.15197585e+00 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5.79200137e+00 5.11075030e+00 4.38625876e+00 3.68432747e+00 + 3.04306956e+00 2.40488805e+00 1.79695778e+00 1.21944967e+00 + 7.56622800e-01 3.53785678e-01 1.14510340e-02 -2.71064775e-01 + -5.71868057e-01 -8.14732524e-01 -1.03025124e+00 -1.20030764e+00 + -1.39446249e+00 -1.66245086e+00 -1.95966501e+00 -2.25619552e+00 + -2.55101692e+00 -2.79165955e+00 -2.98513075e+00 -3.16133994e+00 + -3.27277388e+00 -3.29858144e+00 -3.32473082e+00]",1.0,1.0, +1833-010719-2,False,1833,11.0,True,False,2,ms,True,stim i,5,235,0.20963767864923527,0.26420642903475317,32.22939028477303,"[ 2.34594022 0.8710809 0.19122269 0.72020025 2.49291091 + 4.95490755 7.01158372 7.11156179 3.81111598 -3.52634656 + -15.27541475 -31.37235986 -49.14912665 -64.26975079 -72.96707593 + -72.98593145 -64.02477527 -47.86961924 -28.15888329 -9.85408902 + 4.29437989 13.95611343 18.47255719 18.26788716 15.01523789 + 10.7613462 7.13662939 5.03131623 4.68910327 5.70576628 + 7.44423737 9.21614412 10.52908719 11.12869985 11.04132866 + 10.53219323 9.87959512 9.33380838 9.01257034 8.94442008 + 8.99323979 9.07293722 9.02035372 8.82335745 8.47105898 + 7.99302054 7.44160637 6.88708617 6.36983849 5.8822992 + 5.41836586 4.95856172 4.49627275 4.00931813 3.48717387 + 2.95910984 2.41124023 1.83011766 1.24903165 0.70821457 + 0.20945699 -0.24950669 -0.66794563 -1.03942849 -1.37235986 + -1.64996711 -1.91306731 -2.14298765 -2.35978952 -2.58258423 + -2.80103048 -3.01501864 -3.21179566 -3.40360301 -3.58810202]",0.0,0.0, +1833-010719-2,False,1833,11.0,True,False,2,ms,True,stim i,6,225,0.2890181386872645,0.6031603351913833,26.018113358591165,"[ 6.42617237e+00 6.36221257e+00 6.72668839e+00 7.38891907e+00 + 8.00090531e+00 7.93278110e+00 6.49624298e+00 3.05454463e+00 + -2.70636429e+00 -1.09343654e+01 -2.13223791e+01 -3.27113887e+01 + -4.34244523e+01 -5.19120496e+01 -5.70003169e+01 -5.81096777e+01 + -5.53223339e+01 -4.93159062e+01 -4.11961796e+01 -3.21520460e+01 + -2.32898334e+01 -1.54013217e+01 -8.91268332e+00 -3.74407025e+00 + 4.66458446e-01 4.00810248e+00 7.10524172e+00 9.88018287e+00 + 1.23369093e+01 1.44164856e+01 1.60399240e+01 1.71701521e+01 + 1.77842205e+01 1.79422415e+01 1.77160511e+01 1.72595057e+01 + 1.66587000e+01 1.59831161e+01 1.52715915e+01 1.45334963e+01 + 1.37821836e+01 1.30185588e+01 1.21997556e+01 1.13464150e+01 + 1.04515662e+01 9.53865653e+00 8.62647112e+00 7.74977367e+00 + 6.89281188e+00 6.12217092e+00 5.43336955e+00 4.75190114e+00 + 4.10166576e+00 3.45568532e+00 2.81812421e+00 2.20165671e+00 + 1.59655079e+00 1.03254572e+00 5.03259098e-01 -2.16820569e-02 + -4.77820025e-01 -8.98515300e-01 -1.26946406e+00 -1.62208039e+00 + -1.91671193e+00 -2.21754481e+00 -2.50081477e+00 -2.72542097e+00 + -2.95532319e+00 -3.17522180e+00 -3.37131088e+00 -3.55649104e+00 + -3.70224516e+00 -3.82165490e+00 -3.91218541e+00]",1.0,1.0, +1833-010719-2,False,1833,11.0,True,False,2,ms,True,stim i,7,211,0.2665285790781079,0.674207442158712,2.7605675227474964,"[ 3.37372014e+00 2.72866894e+00 2.26151877e+00 1.99317406e+00 + 1.76237201e+00 1.19197952e+00 -1.74914676e-01 -2.58489761e+00 + -6.24402730e+00 -1.14057167e+01 -1.82444539e+01 -2.61390785e+01 + -3.37218430e+01 -3.95051195e+01 -4.23545222e+01 -4.18476962e+01 + -3.80435154e+01 -3.17073379e+01 -2.39867747e+01 -1.63387372e+01 + -9.91126280e+00 -5.15017065e+00 -1.88950512e+00 2.55546075e-01 + 1.50853242e+00 2.23208191e+00 2.87627986e+00 3.72610922e+00 + 4.83319113e+00 6.19027304e+00 7.63310580e+00 8.96757679e+00 + 1.00123720e+01 1.07239761e+01 1.10170648e+01 1.10328498e+01 + 1.07939420e+01 1.04325939e+01 1.00247440e+01 9.61518771e+00 + 9.30204778e+00 8.94667235e+00 8.63779863e+00 8.31271331e+00 + 7.91168942e+00 7.50213311e+00 7.09982935e+00 6.62201365e+00 + 6.14206485e+00 5.57636519e+00 4.93856655e+00 4.28284983e+00 + 3.66211604e+00 3.00597270e+00 2.42278157e+00 1.91638225e+00 + 1.35836177e+00 9.17662116e-01 6.36092150e-01 4.24914676e-01 + 1.60409556e-01 -3.66894198e-02 -2.11177474e-01 -4.17662116e-01 + -7.53839590e-01 -1.10878840e+00 -1.41979522e+00 -1.73208191e+00 + -2.02986348e+00 -2.26749147e+00 -2.45435154e+00 -2.62755973e+00 + -2.76962457e+00 -2.95093857e+00 -3.13011945e+00]",1.0,1.0, +1833-010719-2,False,1833,11.0,True,False,2,ms,True,stim i,7,213,0.27024899571308136,0.6125346062495727,1.8537258023910237,"[ 3.16836086 2.92947903 3.13786531 3.79161372 4.63151207 + 5.18487929 4.90088945 3.2566709 -0.18932656 -5.50127065 + -12.86721728 -21.58132147 -30.27890724 -37.52287166 -42.08068615 + -43.2433291 -41.03113088 -36.15120712 -29.57687421 -22.37166455 + -15.68170267 -10.09212198 -5.80749682 -2.60419314 -0.10927573 + 2.01905972 4. 6.00317662 8.01207116 9.97331639 + 11.64167726 12.88754765 13.63532402 13.886277 13.76937738 + 13.21855146 12.42884371 11.65374841 10.95298602 10.34053367 + 9.7407878 9.12388818 8.42185515 7.80495553 7.14739517 + 6.48221093 5.80559085 5.29479034 4.82973316 4.47585769 + 4.15819568 3.69695044 3.18487929 2.51778907 1.86531131 + 1.2090216 0.56480305 0.05336722 -0.35578145 -0.63786531 + -0.77318933 -0.90978399 -1.04066074 -1.16327827 -1.41168996 + -1.59275731 -1.84561626 -2.09911055 -2.4021601 -2.63087675 + -2.77636595 -2.94409149 -3.0317662 -3.00444727 -3.02986023]",1.0,1.0, +1833-010719-2,False,1833,11.0,True,False,2,ms,True,stim i,7,217,0.28365331132716765,0.6850618612787205,6.696496132398577,"[ 4.28543792e+00 4.80302497e+00 5.96552937e+00 7.57193106e+00 + 9.16092156e+00 9.96201196e+00 9.23513894e+00 6.30214562e+00 + 9.53570172e-01 -6.74041505e+00 -1.64843475e+01 -2.71000703e+01 + -3.68665142e+01 -4.44254309e+01 -4.86677805e+01 -4.92099894e+01 + -4.62587056e+01 -4.06894126e+01 -3.36686599e+01 -2.64052058e+01 + -1.98485754e+01 -1.43559620e+01 -1.00471333e+01 -6.70260288e+00 + -3.98716145e+00 -1.57791066e+00 7.77875484e-01 3.24340485e+00 + 5.67376011e+00 8.02339078e+00 1.00937390e+01 1.17608160e+01 + 1.29897995e+01 1.37785790e+01 1.41924024e+01 1.43257123e+01 + 1.42805135e+01 1.41229335e+01 1.39416110e+01 1.36850158e+01 + 1.32922969e+01 1.28589518e+01 1.23455856e+01 1.16394654e+01 + 1.08721421e+01 1.00494196e+01 9.23197327e+00 8.40608512e+00 + 7.65265565e+00 6.93105874e+00 6.26081604e+00 5.64808301e+00 + 5.04555047e+00 4.48417165e+00 3.86158987e+00 3.20998945e+00 + 2.54009849e+00 1.93316919e+00 1.36862469e+00 8.65634893e-01 + 4.16109743e-01 3.79880408e-02 -3.08301090e-01 -5.78438269e-01 + -8.68448822e-01 -1.11712979e+00 -1.37671474e+00 -1.59989448e+00 + -1.85860007e+00 -2.14931410e+00 -2.39588463e+00 -2.60253254e+00 + -2.80495955e+00 -2.97485051e+00 -3.19380936e+00]",1.0,1.0, diff --git a/actions/waveform-analysis/data/statistics/broad_spiking.csv b/actions/waveform-analysis/data/statistics/broad_spiking.csv index 5a3cd2872..d7de74966 100644 --- a/actions/waveform-analysis/data/statistics/broad_spiking.csv +++ b/actions/waveform-analysis/data/statistics/broad_spiking.csv @@ -1,4 +1,4 @@ -,Control,Stimulated,MWU,PRS -average_firing_rate,9.53 ± 0.37 (491),10.62 ± 0.44 (462),"104995.00, 0.047","1.09, 0.053" -half_width,0.30 ± 0.00 (491),0.30 ± 0.00 (462),"116089.00, 0.530","0.00, 0.425" -peak_to_trough,0.66 ± 0.00 (491),0.67 ± 0.01 (462),"116944.00, 0.407","0.00, 0.539" +,Control,Stimulated,MWU,PRS +average_firing_rate,10.02 ± 0.37 (478),10.63 ± 0.43 (488),"112628.00, 0.356","0.61, 0.280" +half_width,0.29 ± 0.00 (478),0.30 ± 0.00 (488),"117634.00, 0.817","0.00, 0.610" +peak_to_trough,0.66 ± 0.00 (478),0.66 ± 0.01 (488),"121739.00, 0.239","0.01, 0.487" diff --git a/actions/waveform-analysis/data/statistics/broad_spiking.tex b/actions/waveform-analysis/data/statistics/broad_spiking.tex index 975d17bc5..203da8e12 100644 --- a/actions/waveform-analysis/data/statistics/broad_spiking.tex +++ b/actions/waveform-analysis/data/statistics/broad_spiking.tex @@ -1,9 +1,9 @@ -\begin{tabular}{lllll} -\toprule -{} & Control & Stimulated & MWU & PRS \\ -\midrule -average\_firing\_rate & 9.53 ± 0.37 (491) & 10.62 ± 0.44 (462) & 104995.00, 0.047 & 1.09, 0.053 \\ -half\_width & 0.30 ± 0.00 (491) & 0.30 ± 0.00 (462) & 116089.00, 0.530 & 0.00, 0.425 \\ -peak\_to\_trough & 0.66 ± 0.00 (491) & 0.67 ± 0.01 (462) & 116944.00, 0.407 & 0.00, 0.539 \\ -\bottomrule -\end{tabular} +\begin{tabular}{lllll} +\toprule +{} & Control & Stimulated & MWU & PRS \\ +\midrule +average\_firing\_rate & 10.02 ± 0.37 (478) & 10.63 ± 0.43 (488) & 112628.00, 0.356 & 0.61, 0.280 \\ +half\_width & 0.29 ± 0.00 (478) & 0.30 ± 0.00 (488) & 117634.00, 0.817 & 0.00, 0.610 \\ +peak\_to\_trough & 0.66 ± 0.00 (478) & 0.66 ± 0.01 (488) & 121739.00, 0.239 & 0.01, 0.487 \\ +\bottomrule +\end{tabular} diff --git a/actions/waveform-analysis/data/statistics/broad_spiking_11.csv b/actions/waveform-analysis/data/statistics/broad_spiking_11.csv index 991100368..de74c3f99 100644 --- a/actions/waveform-analysis/data/statistics/broad_spiking_11.csv +++ b/actions/waveform-analysis/data/statistics/broad_spiking_11.csv @@ -1,4 +1,4 @@ -,Control,Stimulated,MWU,PRS -average_firing_rate,9.53 ± 0.37 (491),10.04 ± 0.49 (269),"63173.00, 0.322","0.51, 0.406" -half_width,0.30 ± 0.00 (491),0.30 ± 0.00 (269),"67834.00, 0.535","0.00, 0.655" -peak_to_trough,0.66 ± 0.00 (491),0.67 ± 0.01 (269),"66266.00, 0.938","0.01, 0.279" +,Control,Stimulated,MWU,PRS +average_firing_rate,10.02 ± 0.37 (478),10.15 ± 0.47 (294),"69504.00, 0.800","0.13, 0.832" +half_width,0.29 ± 0.00 (478),0.30 ± 0.00 (294),"71749.00, 0.622","0.00, 0.563" +peak_to_trough,0.66 ± 0.00 (478),0.67 ± 0.01 (294),"72358.50, 0.487","0.01, 0.329" diff --git a/actions/waveform-analysis/data/statistics/broad_spiking_11.tex b/actions/waveform-analysis/data/statistics/broad_spiking_11.tex index fe8f8d2d4..3ca943de8 100644 --- a/actions/waveform-analysis/data/statistics/broad_spiking_11.tex +++ b/actions/waveform-analysis/data/statistics/broad_spiking_11.tex @@ -1,9 +1,9 @@ -\begin{tabular}{lllll} -\toprule -{} & Control & Stimulated & MWU & PRS \\ -\midrule -average\_firing\_rate & 9.53 ± 0.37 (491) & 10.04 ± 0.49 (269) & 63173.00, 0.322 & 0.51, 0.406 \\ -half\_width & 0.30 ± 0.00 (491) & 0.30 ± 0.00 (269) & 67834.00, 0.535 & 0.00, 0.655 \\ -peak\_to\_trough & 0.66 ± 0.00 (491) & 0.67 ± 0.01 (269) & 66266.00, 0.938 & 0.01, 0.279 \\ -\bottomrule -\end{tabular} +\begin{tabular}{lllll} +\toprule +{} & Control & Stimulated & MWU & PRS \\ +\midrule +average\_firing\_rate & 10.02 ± 0.37 (478) & 10.15 ± 0.47 (294) & 69504.00, 0.800 & 0.13, 0.832 \\ +half\_width & 0.29 ± 0.00 (478) & 0.30 ± 0.00 (294) & 71749.00, 0.622 & 0.00, 0.563 \\ +peak\_to\_trough & 0.66 ± 0.00 (478) & 0.67 ± 0.01 (294) & 72358.50, 0.487 & 0.01, 0.329 \\ +\bottomrule +\end{tabular} diff --git a/actions/waveform-analysis/data/statistics/broad_spiking_30.csv b/actions/waveform-analysis/data/statistics/broad_spiking_30.csv index 9145f6688..87950188f 100644 --- a/actions/waveform-analysis/data/statistics/broad_spiking_30.csv +++ b/actions/waveform-analysis/data/statistics/broad_spiking_30.csv @@ -1,4 +1,4 @@ -,Control,Stimulated,MWU,PRS -average_firing_rate,9.53 ± 0.37 (491),11.42 ± 0.81 (193),"41822.00, 0.017","1.89, 0.013" -half_width,0.30 ± 0.00 (491),0.29 ± 0.01 (193),"48255.00, 0.707","0.01, 0.384" -peak_to_trough,0.66 ± 0.00 (491),0.66 ± 0.01 (193),"50678.00, 0.156","0.00, 0.854" +,Control,Stimulated,MWU,PRS +average_firing_rate,10.02 ± 0.37 (478),11.36 ± 0.80 (194),"43124.00, 0.155","1.34, 0.085" +half_width,0.29 ± 0.00 (478),0.29 ± 0.01 (194),"45885.00, 0.833","0.00, 0.804" +peak_to_trough,0.66 ± 0.00 (478),0.66 ± 0.01 (194),"49380.50, 0.186","0.00, 0.920" diff --git a/actions/waveform-analysis/data/statistics/broad_spiking_30.tex b/actions/waveform-analysis/data/statistics/broad_spiking_30.tex index 29a379345..3d9f44dd4 100644 --- a/actions/waveform-analysis/data/statistics/broad_spiking_30.tex +++ b/actions/waveform-analysis/data/statistics/broad_spiking_30.tex @@ -1,9 +1,9 @@ -\begin{tabular}{lllll} -\toprule -{} & Control & Stimulated & MWU & PRS \\ -\midrule -average\_firing\_rate & 9.53 ± 0.37 (491) & 11.42 ± 0.81 (193) & 41822.00, 0.017 & 1.89, 0.013 \\ -half\_width & 0.30 ± 0.00 (491) & 0.29 ± 0.01 (193) & 48255.00, 0.707 & 0.01, 0.384 \\ -peak\_to\_trough & 0.66 ± 0.00 (491) & 0.66 ± 0.01 (193) & 50678.00, 0.156 & 0.00, 0.854 \\ -\bottomrule -\end{tabular} +\begin{tabular}{lllll} +\toprule +{} & Control & Stimulated & MWU & PRS \\ +\midrule +average\_firing\_rate & 10.02 ± 0.37 (478) & 11.36 ± 0.80 (194) & 43124.00, 0.155 & 1.34, 0.085 \\ +half\_width & 0.29 ± 0.00 (478) & 0.29 ± 0.01 (194) & 45885.00, 0.833 & 0.00, 0.804 \\ +peak\_to\_trough & 0.66 ± 0.00 (478) & 0.66 ± 0.01 (194) & 49380.50, 0.186 & 0.00, 0.920 \\ +\bottomrule +\end{tabular} diff --git a/actions/waveform-analysis/data/statistics/narrow_spiking.csv b/actions/waveform-analysis/data/statistics/narrow_spiking.csv index 06d3ed335..c72c285f2 100644 --- a/actions/waveform-analysis/data/statistics/narrow_spiking.csv +++ b/actions/waveform-analysis/data/statistics/narrow_spiking.csv @@ -1,4 +1,4 @@ -,Control,Stimulated,MWU,PRS -average_firing_rate,21.22 ± 1.17 (147),20.20 ± 0.96 (178),"13472.00, 0.645","1.02, 0.494" -half_width,0.23 ± 0.01 (147),0.23 ± 0.00 (178),"12839.00, 0.773","0.01, 0.456" -peak_to_trough,0.29 ± 0.00 (147),0.29 ± 0.00 (178),"13194.50, 0.895","0.00, 0.892" +,Control,Stimulated,MWU,PRS +average_firing_rate,21.83 ± 1.15 (145),20.79 ± 0.92 (184),"13743.00, 0.638","1.04, 0.470" +half_width,0.23 ± 0.01 (145),0.23 ± 0.00 (184),"12714.00, 0.465","0.00, 0.720" +peak_to_trough,0.29 ± 0.00 (145),0.29 ± 0.00 (184),"13281.00, 0.946","0.00, 0.839" diff --git a/actions/waveform-analysis/data/statistics/narrow_spiking.tex b/actions/waveform-analysis/data/statistics/narrow_spiking.tex index 049dfab3c..e61bfa646 100644 --- a/actions/waveform-analysis/data/statistics/narrow_spiking.tex +++ b/actions/waveform-analysis/data/statistics/narrow_spiking.tex @@ -1,9 +1,9 @@ -\begin{tabular}{lllll} -\toprule -{} & Control & Stimulated & MWU & PRS \\ -\midrule -average\_firing\_rate & 21.22 ± 1.17 (147) & 20.20 ± 0.96 (178) & 13472.00, 0.645 & 1.02, 0.494 \\ -half\_width & 0.23 ± 0.01 (147) & 0.23 ± 0.00 (178) & 12839.00, 0.773 & 0.01, 0.456 \\ -peak\_to\_trough & 0.29 ± 0.00 (147) & 0.29 ± 0.00 (178) & 13194.50, 0.895 & 0.00, 0.892 \\ -\bottomrule -\end{tabular} +\begin{tabular}{lllll} +\toprule +{} & Control & Stimulated & MWU & PRS \\ +\midrule +average\_firing\_rate & 21.83 ± 1.15 (145) & 20.79 ± 0.92 (184) & 13743.00, 0.638 & 1.04, 0.470 \\ +half\_width & 0.23 ± 0.01 (145) & 0.23 ± 0.00 (184) & 12714.00, 0.465 & 0.00, 0.720 \\ +peak\_to\_trough & 0.29 ± 0.00 (145) & 0.29 ± 0.00 (184) & 13281.00, 0.946 & 0.00, 0.839 \\ +\bottomrule +\end{tabular} diff --git a/actions/waveform-analysis/data/statistics/narrow_spiking_11.csv b/actions/waveform-analysis/data/statistics/narrow_spiking_11.csv index 6d145a175..0b3910055 100644 --- a/actions/waveform-analysis/data/statistics/narrow_spiking_11.csv +++ b/actions/waveform-analysis/data/statistics/narrow_spiking_11.csv @@ -1,4 +1,4 @@ -,Control,Stimulated,MWU,PRS -average_firing_rate,21.22 ± 1.17 (147),19.13 ± 1.31 (102),"8043.00, 0.329","2.08, 0.236" -half_width,0.23 ± 0.01 (147),0.23 ± 0.00 (102),"7299.00, 0.724","0.01, 0.299" -peak_to_trough,0.29 ± 0.00 (147),0.29 ± 0.00 (102),"7471.50, 0.964","0.00, 0.814" +,Control,Stimulated,MWU,PRS +average_firing_rate,21.83 ± 1.15 (145),19.85 ± 1.22 (110),"8502.00, 0.367","1.98, 0.244" +half_width,0.23 ± 0.01 (145),0.23 ± 0.00 (110),"7419.00, 0.341","0.00, 0.547" +peak_to_trough,0.29 ± 0.00 (145),0.29 ± 0.00 (110),"7711.50, 0.652","0.00, 0.696" diff --git a/actions/waveform-analysis/data/statistics/narrow_spiking_11.tex b/actions/waveform-analysis/data/statistics/narrow_spiking_11.tex index ffa32fa32..fc4f437cc 100644 --- a/actions/waveform-analysis/data/statistics/narrow_spiking_11.tex +++ b/actions/waveform-analysis/data/statistics/narrow_spiking_11.tex @@ -1,9 +1,9 @@ -\begin{tabular}{lllll} -\toprule -{} & Control & Stimulated & MWU & PRS \\ -\midrule -average\_firing\_rate & 21.22 ± 1.17 (147) & 19.13 ± 1.31 (102) & 8043.00, 0.329 & 2.08, 0.236 \\ -half\_width & 0.23 ± 0.01 (147) & 0.23 ± 0.00 (102) & 7299.00, 0.724 & 0.01, 0.299 \\ -peak\_to\_trough & 0.29 ± 0.00 (147) & 0.29 ± 0.00 (102) & 7471.50, 0.964 & 0.00, 0.814 \\ -\bottomrule -\end{tabular} +\begin{tabular}{lllll} +\toprule +{} & Control & Stimulated & MWU & PRS \\ +\midrule +average\_firing\_rate & 21.83 ± 1.15 (145) & 19.85 ± 1.22 (110) & 8502.00, 0.367 & 1.98, 0.244 \\ +half\_width & 0.23 ± 0.01 (145) & 0.23 ± 0.00 (110) & 7419.00, 0.341 & 0.00, 0.547 \\ +peak\_to\_trough & 0.29 ± 0.00 (145) & 0.29 ± 0.00 (110) & 7711.50, 0.652 & 0.00, 0.696 \\ +\bottomrule +\end{tabular} diff --git a/actions/waveform-analysis/data/statistics/narrow_spiking_30.csv b/actions/waveform-analysis/data/statistics/narrow_spiking_30.csv index e7e7077a1..a9d0c9847 100644 --- a/actions/waveform-analysis/data/statistics/narrow_spiking_30.csv +++ b/actions/waveform-analysis/data/statistics/narrow_spiking_30.csv @@ -1,4 +1,4 @@ -,Control,Stimulated,MWU,PRS -average_firing_rate,21.22 ± 1.17 (147),21.62 ± 1.40 (76),"5429.00, 0.732","0.41, 0.822" -half_width,0.23 ± 0.01 (147),0.23 ± 0.01 (76),"5540.00, 0.921","0.00, 0.846" -peak_to_trough,0.29 ± 0.00 (147),0.29 ± 0.01 (76),"5723.00, 0.765","0.00, 0.582" +,Control,Stimulated,MWU,PRS +average_firing_rate,21.83 ± 1.15 (145),22.18 ± 1.39 (74),"5241.00, 0.781","0.35, 0.860" +half_width,0.23 ± 0.01 (145),0.23 ± 0.01 (74),"5295.00, 0.875","0.00, 0.998" +peak_to_trough,0.29 ± 0.00 (145),0.28 ± 0.00 (74),"5569.50, 0.646","0.01, 0.355" diff --git a/actions/waveform-analysis/data/statistics/narrow_spiking_30.tex b/actions/waveform-analysis/data/statistics/narrow_spiking_30.tex index f48a8f457..4bfd161b1 100644 --- a/actions/waveform-analysis/data/statistics/narrow_spiking_30.tex +++ b/actions/waveform-analysis/data/statistics/narrow_spiking_30.tex @@ -1,9 +1,9 @@ -\begin{tabular}{lllll} -\toprule -{} & Control & Stimulated & MWU & PRS \\ -\midrule -average\_firing\_rate & 21.22 ± 1.17 (147) & 21.62 ± 1.40 (76) & 5429.00, 0.732 & 0.41, 0.822 \\ -half\_width & 0.23 ± 0.01 (147) & 0.23 ± 0.01 (76) & 5540.00, 0.921 & 0.00, 0.846 \\ -peak\_to\_trough & 0.29 ± 0.00 (147) & 0.29 ± 0.01 (76) & 5723.00, 0.765 & 0.00, 0.582 \\ -\bottomrule -\end{tabular} +\begin{tabular}{lllll} +\toprule +{} & Control & Stimulated & MWU & PRS \\ +\midrule +average\_firing\_rate & 21.83 ± 1.15 (145) & 22.18 ± 1.39 (74) & 5241.00, 0.781 & 0.35, 0.860 \\ +half\_width & 0.23 ± 0.01 (145) & 0.23 ± 0.01 (74) & 5295.00, 0.875 & 0.00, 0.998 \\ +peak\_to\_trough & 0.29 ± 0.00 (145) & 0.28 ± 0.00 (74) & 5569.50, 0.646 & 0.01, 0.355 \\ +\bottomrule +\end{tabular}